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Applied and Environmental Microbiology, October 2002, p. 5170-5176, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5170-5176.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification of Differences in Genome Content among phlD-Positive Pseudomonas fluorescens Strains by Using PCR-Based Subtractive Hybridization

D. V. Mavrodi,1,2* O. V. Mavrodi,1 B. B. McSpadden-Gardener,2 B. B. Landa,1 D. M. Weller,1,2 and L. S. Thomashow1,2,3

Department of Plant Pathology,1 School of Molecular Biosciences, Washington State University,2 Root Disease and Biological Control Research Unit, USDA Agricultural Research Service, Pullman, Washington3

Received 18 March 2002/ Accepted 17 July 2002

Certain 2,4-diacetylphloroglucinol-producing strains of Pseudomonas fluorescens colonize roots and suppress soilborne diseases more effectively than others from which they are otherwise phenotypically almost indistinguishable. We recovered DNA fragments present in the superior colonizer P. fluorescens Q8r1-96 but not in the less rhizosphere-competent strain Q2-87. Of the open reading frames in 32 independent Q8r1-96-specific clones, 1 was similar to colicin M from Escherichia coli, 3 resembled known regulatory proteins, and 28 had no significant match with sequences of known function. Seven clones hybridized preferentially to DNA from strains with superior rhizosphere competence, and sequences in two others were highly expressed in vitro and in the rhizosphere.

* Corresponding author. Mailing address: USDA-ARS Root Disease & Biological Control Research Unit, Washington State University, Pullman, WA 99164-6430. Phone: (509) 335-3269. Fax: (509) 335-7674. E-mail: mavrodi{at}mail.wsu.edu.

Applied and Environmental Microbiology, October 2002, p. 5170-5176, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5170-5176.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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