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Applied and Environmental Microbiology, October 2002, p. 5191-5194, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5191-5194.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Carbon and Hydrogen Stable Isotope Fractionation during Aerobic Bacterial Degradation of Aromatic Hydrocarbons{dagger}

Barbara Morasch,1,{ddagger} Hans H. Richnow,2 Bernhard Schink,1 Andrea Vieth,2 and Rainer U. Meckenstock1,{ddagger}*

Fakultät für Biologie, Universität Konstanz, D-78457 Konstanz,1 Umweltforschungszentrum Leipzig-Halle GmbH, D-04318 Leipzig, Germany2

Received 23 January 2002/ Accepted 1 July 2002

13C/12C and D/H stable isotope fractionation during aerobic degradation was determined for Pseudomonas putida strain mt-2, Pseudomonas putida strain F1, Ralstonia pickettii strain PKO1, and Pseudomonas putida strain NCIB 9816 grown with toluene, xylenes, and naphthalene. Different types of initial reactions used by the respective bacterial strains could be linked with certain extents of stable isotope fractionation during substrate degradation.

* Corresponding author. Present address: Zentrum für Angewandte Geowissenschaften, Eberhard-Karls-Universität Tübingen, Sigwartstr. 10, D-72076 Tübingen, Germany. Phone: 49-(0)7071-2973150. Fax: 49-(0)7071-295139. E-mail: rainer.meckenstock{at}uni-tuebingen.de.

{dagger} This paper represents publication no. 182 of the Deutsche Forschungsgemeinschaft priority program 546 "Geochemical processes with long-term effects in anthropogenically affected seepage- and groundwater."

{ddagger} Present address: Zentrum für Angewandte Geowissenschaften, Eberhard-Karls-Universität Tübingen, D-72076 Tübingen, Germany.

Applied and Environmental Microbiology, October 2002, p. 5191-5194, Vol. 68, No. 10
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.10.5191-5194.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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