This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cristescu, S. M.
Right arrow Articles by Harren, F. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cristescu, S. M.
Right arrow Articles by Harren, F. J. M.
Agricola
Right arrow Articles by Cristescu, S. M.
Right arrow Articles by Harren, F. J. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2002, p. 5342-5350, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5342-5350.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ethylene Production by Botrytis cinerea In Vitro and in Tomatoes

Simona M. Cristescu,1* Domenico De Martinis,2 Sacco te Lintel Hekkert,1 David H. Parker,1 and Frans J. M. Harren1

Department of Molecular and Laser Physics, University of Nijmegen, 6525 ED Nijmegen, The Netherlands,1 ENEA, UTS Biotechnology, Protection of Human Health and Ecosystem, Section of Plant Genetics and Genomics, C.R. Casaccia, 00060 Rome, Italy2

Received 10 April 2002/ Accepted 31 July 2002

A laser-based ethylene detector was used for on-line monitoring of ethylene released by the phytopathogenic fungus Botrytis cinerea in vitro and in tomato fruit. Ethylene data were combined with the results of a cytological analysis of germination of B. cinerea conidia and hyphal growth. We found that aminoethoxyvinylglycine and aminooxyacetic acid, which are competitive inhibitors of the 1-aminocyclopropane-1-carboxylic acid pathway, did not inhibit the ethylene emission by B. cinerea and that the fungus most likely produces ethylene via the 2-keto-4-methylthiobutyric acid pathway. B. cinerea is able to produce ethylene in vitro, and the emission of ethylene follows the pattern that is associated with hyphal growth rather than the germination of conidia. Ethylene production in vitro depended on the L-methionine concentration added to the plating medium. Higher values and higher emission rates were observed when the concentration of conidia was increased. Compared with the ethylene released by the fungus, the infection-related ethylene produced by two tomato cultivars (cultivars Money Maker and Daniela) followed a similar pattern, but the levels of emission were 100-fold higher. The time evolution of enhanced ethylene production by the infected tomatoes and the cytological observations indicate that ethylene emission by the tomato-fungus system is not triggered by the ethylene produced by B. cinerea, although it is strongly synchronized with the growth rate of the fungus inside the tomato.

* Corresponding author. Mailing address: Department of Molecular and Laser Physics, University of Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands. Phone: 31-24-3652338. Fax: 31-24-3653311. E-mail: simona{at}sci.kun.nl.

Applied and Environmental Microbiology, November 2002, p. 5342-5350, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5342-5350.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Splivallo, R., Fischer, U., Gobel, C., Feussner, I., Karlovsky, P. (2009). Truffles Regulate Plant Root Morphogenesis via the Production of Auxin and Ethylene. Plant Physiol. 150: 2018-2029 [Abstract] [Full Text]  
  • Cantu, D., Blanco-Ulate, B., Yang, L., Labavitch, J. M., Bennett, A. B., Powell, A. L.T. (2009). Ripening-Regulated Susceptibility of Tomato Fruit to Botrytis cinerea Requires NOR But Not RIN or Ethylene. Plant Physiol. 150: 1434-1449 [Abstract] [Full Text]  
  • Mur, L. A.J., Laarhoven, L. J.J., Harren, F. J.M., Hall, M. A., Smith, A. R. (2008). Nitric Oxide Interacts with Salicylate to Regulate Biphasic Ethylene Production during the Hypersensitive Response. Plant Physiol. 148: 1537-1546 [Abstract] [Full Text]  
  • Sun, H.-J., Uchii, S., Watanabe, S., Ezura, H. (2006). A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics. Plant Cell Physiol 47: 426-431 [Abstract] [Full Text]  
  • Mur, L. A.J., Santosa, I. E., Laarhoven, L. J.J., Holton, N. J., Harren, F. J.M., Smith, A. R. (2005). Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars. Plant Physiol. 138: 1247-1258 [Abstract] [Full Text]  
  • Montero, C., Cristescu, S.M., Jimenez, J.B., Orea, J.M., te Lintel Hekkert, S., Harren, F.J.M., Gonzalez Urena, A. (2003). trans-Resveratrol and Grape Disease Resistance. A Dynamical Study by High-Resolution Laser-Based Techniques. Plant Physiol. 131: 129-138 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»