This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, J.
Right arrow Articles by Kaletunç, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, J.
Right arrow Articles by Kaletunç, G.
Agricola
Right arrow Articles by Lee, J.
Right arrow Articles by Kaletunç, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2002, p. 5379-5386, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5379-5386.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Evaluation of the Heat Inactivation of Escherichia coli and Lactobacillus plantarum by Differential Scanning Calorimetry

Jaesung Lee and Gönül Kaletunç*

Department of Food, Agricultural, and Biological Engineering, The Ohio State University, Columbus, Ohio

Received 19 February 2002/ Accepted 29 July 2002

Differential scanning calorimetry (DSC) is used to evaluate the thermal stability and reversibility after heat treatment of transitions associated with various cellular components of Escherichia coli and Lactobacillus plantarum. The reversibility and the change in the thermal stability of individual transitions are evaluated by a second temperature scan after preheating in the DSC to various temperatures between 40 and 130°C. The viability of bacteria after a heat treatment between 55 and 70°C in the DSC is determined by both plate count and calorimetric data. The fractional viability values based on calorimetric and plate count data show a linear relationship. Viability loss and the irreversible change in DSC thermograms of pretreated whole cells are highly correlated between 55 and 70°C. Comparison of DSC scans for isolated ribosomes shows that the thermal stability of E. coli ribosomes is greater than that of L. plantarum ribosomes, consistent with the greater thermal tolerance of E. coli observed from viability loss and DSC scans of whole cells.

* Corresponding author. Mailing address: Department of Food, Agricultural and Biological Engineering, The Ohio State University, 590 Woody Hayes Dr., Columbus, OH 43210. Phone: (614) 292-0419. Fax: (614) 292-9448. E-mail: kaletunc.1{at}osu.edu.

Applied and Environmental Microbiology, November 2002, p. 5379-5386, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5379-5386.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Berney, M., Weilenmann, H.-U., Ihssen, J., Bassin, C., Egli, T. (2006). Specific Growth Rate Determines the Sensitivity of Escherichia coli to Thermal, UVA, and Solar Disinfection. Appl. Environ. Microbiol. 72: 2586-2593 [Abstract] [Full Text]  
  • Nguyen, H. T. T., Corry, J. E. L., Miles, C. A. (2006). Heat Resistance and Mechanism of Heat Inactivation in Thermophilic Campylobacters. Appl. Environ. Microbiol. 72: 908-913 [Abstract] [Full Text]  
  • De Angelis, M., Di Cagno, R., Huet, C., Crecchio, C., Fox, P. F., Gobbetti, M. (2004). Heat Shock Response in Lactobacillus plantarum. Appl. Environ. Microbiol. 70: 1336-1346 [Abstract] [Full Text]  
  • Kaletunc, G., Lee, J., Alpas, H., Bozoglu, F. (2004). Evaluation of Structural Changes Induced by High Hydrostatic Pressure in Leuconostoc mesenteroides. Appl. Environ. Microbiol. 70: 1116-1122 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»