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Applied and Environmental Microbiology, November 2002, p. 5417-5421, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5417-5421.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Pine Oil Cleaner-Resistant Staphylococcus aureus: Reduced Susceptibility to Vancomycin and Oxacillin and Involvement of SigB

Christopher T. D. Price,1,2 Vineet K. Singh,3 Radheshyam K. Jayaswal,3 Brian J. Wilkinson,3 and John E. Gustafson1,2*

Microbiology Department, College of Osteopathic Medicine, Midwestern University, Downers Grove, Illinois 60515,1 School of Biomedical Sciences, Curtin University of Technology, Perth, Western Australia 6845, Australia,2 Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, Illinois 61790-41203

Received 13 May 2002/ Accepted 27 August 2002

Mutants of Staphylococcus aureus strain COL resistant to a household pine oil cleaner (POC) were isolated on laboratory media containing POC. S. aureus mutants expressing the POC resistance (POCr) phenotype also demonstrate reduced susceptibility to the cell wall-active antibiotics vancomycin and oxacillin. The POCr phenotype is reliant on the S. aureus alternative transcription factor SigB, since inactivation of sigB abolished expression of elevated POC resistance and the reductions in vancomycin and oxacillin susceptibilities. The isolation of suppressor mutants of COLsigB::kan, which maintain the sigB::kan allele, indicates that the POCr phenotype can also be expressed to a lesser degree via a sigB-independent mechanism. These results bolster a growing body of reports suggesting that common disinfectants can select for bacteria with reduced susceptibilities to antibiotics. A series of in vitro-selected glycopeptide-intermediate S. aureus (GISA) isolates also expressed reductions in POC susceptibility compared to parent strains. Viewed collectively, our evidence suggests that mutations leading to the POCr phenotype may also be involved with the mechanism that leads to the GISA phenotype.

* Corresponding author. Mailing address: Department of Biology, MSC 3AF, New Mexico State University, P.O. Box 30001, Las Cruces, NM 88003-8001. Phone: (505) 646-5660. Fax: (505) 646-5665. E-mail: jgustafs{at}nmsu.edu.

Applied and Environmental Microbiology, November 2002, p. 5417-5421, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5417-5421.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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