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Applied and Environmental Microbiology, November 2002, p. 5437-5444, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5437-5444.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

SED1 Gene Length and Sequence Polymorphisms in Feral Strains of Saccharomyces cerevisiae

Ilaria Mannazzu,* Emanuela Simonetti, Paola Marinangeli, Emanuela Guerra,{dagger} Marilena Budroni,{ddagger} Madan Thangavelu, and Francesca Clementi

Dipartimento di Biotecnologie Agrarie ed Ambientali, Università di Ancona, Ancona, Italy

Received 13 May 2002/ Accepted 2 August 2002

The SED1 gene (YDR077W), coding for the major cell wall glycoprotein of Saccharomyces cerevisiae stationary-phase cells, contains two blocks of tandem repeat units located within two distinct regions of the nucleotide sequence. A PCR survey of the SED1 open reading frames (ORFs) of 186 previously uncharacterized grape must isolates of S. cerevisiae yielded 13 PCR profiles arising from different combinations of seven SED1 length variants in individuals homozygous or heterozygous for the gene. Comparison of the nucleotide sequences of a group of representatives of each of the seven length variants with those of S288C and the type strain, CBS1171, unequivocally identified them as SED1 alleles and provided evidence for the presence of two minisatellite-like sequences, variable in length, within the ORF of an S. cerevisiae gene. The segregation analyses of the SED1 length variants and other genetic markers in 13 isolates representative of each PCR profile suggested that molecular mechanisms involved in minisatellite expansion and contraction may be responsible for SED1 heterozygosities within a population of homothallic must isolates of S. cerevisiae.

* Corresponding author. Mailing address: Dipartimento di Biotecnologie Agrarie ed Ambientali, Università degli Studi di Ancona, Via Brecce Bianche 60131, Ancona, Italy. Phone: 31 071 2204782. Fax: 31 071 2204858. E-mail: ilaria{at}popcsi.unian.it.

{dagger} Present address: Dipartimento di Biologia Cellulare ed Oncologia, Consorzio M. Negri Sud, Chieti, Italy.

{ddagger} Present address: Dipartimento di Scienze Ambientali Agrarie e Biotecnologie Agro-Alimentari, Università degli Studi di Sassari, Sassari, Italy.

Applied and Environmental Microbiology, November 2002, p. 5437-5444, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5437-5444.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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