This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bakan, B.
Right arrow Articles by Brygoo, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bakan, B.
Right arrow Articles by Brygoo, Y.
Agricola
Right arrow Articles by Bakan, B.
Right arrow Articles by Brygoo, Y.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2002, p. 5472-5479, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5472-5479.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Identification by PCR of Fusarium culmorum Strains Producing Large and Small Amounts of Deoxynivalenol

B. Bakan,1* C. Giraud-Delville,2 L. Pinson,1 D. Richard-Molard,1 E. Fournier,2 and Y. Brygoo2

Laboratoire de Microbiologie et Technologie Céréalières, Institut National de la Recherche Agronomique, 44316 Nantes,1 Laboratoire de Phytopathologie et Méthodologie de la Détection, Institut National de la Recherche Agronomique, 78026 Versailles, France2

Received 18 March 2002/ Accepted 19 August 2002

Thirty deoxynivalenol-producing F. culmorum strains, isolated from wheat grains, were incubated in vitro and analyzed for trichothecene production. Seventeen strains produced more than 1 ppm of deoxynivalenol and acetyldeoxynivalenol and were considered high-deoxynivalenol-producing strains, whereas 13 F. culmorum strains produced less than 0.07 ppm of trichothecenes and were considered low-deoxynivalenol-producing strains. For all strains, a 550-base portion of the trichodiene synthase gene (tri5) was amplified and sequenced. According to the tri5 data, the F. culmorum strains tested clustered into two groups that correlated with in vitro deoxynivalenol production. For three high-producing and three low-producing F. culmorum strains, the tri5-tri6 intergenic region was then sequenced, which confirmed the two separate clusters within the F. culmorum strains. According to the tri5-tri6 sequence data, specific PCR primers were designed to allow differentiation of high-producing from low-producing F. culmorum strains.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Technologie Céréalières, Institut National de la Recherche Agronomique, 44316 Nantes, France. Phone: 33 2 40 67 52 15. Fax: 33 2 40 67 52 25. E-mail: bakan{at}nantes.inra.fr.

Applied and Environmental Microbiology, November 2002, p. 5472-5479, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5472-5479.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»