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Applied and Environmental Microbiology, November 2002, p. 5750-5755, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5750-5755.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Large-Scale Production of Coenzyme F420-5,6 by Using Mycobacterium smegmatis

Dale Isabelle, D. Randall Simpson, and Lacy Daniels*

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242

Received 7 March 2002/ Accepted 30 July 2002

Production of coenzyme F420 and its biosynthetic precursor FO was examined with a variety of aerobic actinomycetes to identify an improved source for these materials. Based on fermentation costs, safety, and ease of growth, Mycobacterium smegmatis was the best source for F420-5,6. M. smegmatis produced 1 to 3 µmol of intracellular F420 per liter of culture, which was more than the 0.85 to 1.0 µmol of F420-2 per liter usually obtained with Methanobacterium thermoautotrophicum and ~10-fold higher than what was previously reported for the best aerobic actinomycetes. An improved chromatography system using rapidly flowing quaternary aminoethyl ion-exchange material and Florisil was used to more quickly and easily purify F420 than with previous methods.

* Corresponding author. Mailing address: Department of Microbiology, University of Iowa, Iowa City, IA 52242. Phone: (319) 335-7780. Fax: (319) 335-9006. E-mail: lacy-daniels{at}uiowa.edu.

Applied and Environmental Microbiology, November 2002, p. 5750-5755, Vol. 68, No. 11
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.11.5750-5755.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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  • Bashiri, G., Squire, C. J., Moreland, N. J., Baker, E. N. (2008). Crystal Structures of F420-dependent Glucose-6-phosphate Dehydrogenase FGD1 Involved in the Activation of the Anti-tuberculosis Drug Candidate PA-824 Reveal the Basis of Coenzyme and Substrate Binding. J. Biol. Chem. 283: 17531-17541 [Abstract] [Full Text]  
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