This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Casadei, M. A.
Right arrow Articles by Mackey, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casadei, M. A.
Right arrow Articles by Mackey, B. M.
Agricola
Right arrow Articles by Casadei, M. A.
Right arrow Articles by Mackey, B. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2002, p. 5965-5972, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5965-5972.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Membrane Fluidity in Pressure Resistance of Escherichia coli NCTC 8164

M. A. Casadei,{dagger} P. Mañas, G. Niven, E. Needs, and B. M. Mackey*

School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, United Kingdom

Received 6 May 2002/ Accepted 16 September 2002

The relationship among growth temperature, membrane fatty acid composition, and pressure resistance was examined in Escherichia coli NCTC 8164. The pressure resistance of exponential-phase cells was maximal in cells grown at 10°C and decreased with increasing growth temperatures up to 45°C. By contrast, the pressure resistance of stationary-phase cells was lowest in cells grown at 10°C and increased with increasing growth temperature, reaching a maximum at 30 to 37°C before decreasing at 45°C. The proportion of unsaturated fatty acids in the membrane lipids decreased with increasing growth temperature in both exponential- and stationary-phase cells and correlated closely with the melting point of the phospholipids extracted from whole cells examined by differential scanning calorimetry. Therefore, in exponential-phase cells, pressure resistance increased with greater membrane fluidity, whereas in stationary-phase cells, there was apparently no simple relationship between membrane fluidity and pressure resistance. When exponential-phase or stationary-phase cells were pressure treated at different temperatures, resistance in both cell types increased with increasing temperatures of pressurization (between 10 and 30°C). Based on the above observations, we propose that membrane fluidity affects the pressure resistance of exponential- and stationary-phase cells in a similar way, but it is the dominant factor in exponential-phase cells whereas in stationary-phase cells, its effects are superimposed on a separate but larger effect of the physiological stationary-phase response that is itself temperature dependent.

* Corresponding author. Mailing address: School of Food Biosciences, University of Reading, P.O. Box 226, Whiteknights, Reading RG6 6AP, United Kingdom. Phone: 44 1189 357 229. Fax: 44 1189 357 222. E-mail: b.m.mackey{at}reading.ac.uk.

{dagger} Present address: Via Rocca 27, Meldola, 47014 Forli, Italy.

Applied and Environmental Microbiology, December 2002, p. 5965-5972, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.5965-5972.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hughes, R.-A., Hallett, K., Cogan, T., Enser, M., Humphrey, T. (2009). The Response of Campylobacter jejuni to Low Temperature Differs from That of Escherichia coli. Appl. Environ. Microbiol. 75: 6292-6298 [Abstract] [Full Text]  
  • Ozcan, N., Ejsing, C. S., Shevchenko, A., Lipski, A., Morbach, S., Kramer, R. (2007). Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum. J. Bacteriol. 189: 7485-7496 [Abstract] [Full Text]  
  • Klotz, B., Pyle, D. L., Mackey, B. M. (2007). New Mathematical Modeling Approach for Predicting Microbial Inactivation by High Hydrostatic Pressure. Appl. Environ. Microbiol. 73: 2468-2478 [Abstract] [Full Text]  
  • Black, E. P., Koziol-Dube, K., Guan, D., Wei, J., Setlow, B., Cortezzo, D. E., Hoover, D. G., Setlow, P. (2005). Factors Influencing Germination of Bacillus subtilis Spores via Activation of Nutrient Receptors by High Pressure. Appl. Environ. Microbiol. 71: 5879-5887 [Abstract] [Full Text]  
  • Aertsen, A., De Spiegeleer, P., Vanoirbeek, K., Lavilla, M., Michiels, C. W. (2005). Induction of Oxidative Stress by High Hydrostatic Pressure in Escherichia coli. Appl. Environ. Microbiol. 71: 2226-2231 [Abstract] [Full Text]  
  • Murtazina, D. A., Andersson, U., Hahn, I.-S., Bjorkhem, I., Ansari, G. A. S., Pikuleva, I. A. (2004). Phospholipids modify substrate binding and enzyme activity of human cytochrome P450 27A1. J. Lipid Res. 45: 2345-2353 [Abstract] [Full Text]  
  • Yuk, H.-G., Marshall, D. L. (2004). Adaptation of Escherichia coli O157:H7 to pH Alters Membrane Lipid Composition, Verotoxin Secretion, and Resistance to Simulated Gastric Fluid Acid. Appl. Environ. Microbiol. 70: 3500-3505 [Abstract] [Full Text]  
  • Molina-Hoppner, A., Doster, W., Vogel, R. F., Ganzle, M. G. (2004). Protective Effect of Sucrose and Sodium Chloride for Lactococcus lactis during Sublethal and Lethal High-Pressure Treatments. Appl. Environ. Microbiol. 70: 2013-2020 [Abstract] [Full Text]  
  • Manas, P., Mackey, B. M. (2004). Morphological and Physiological Changes Induced by High Hydrostatic Pressure in Exponential- and Stationary-Phase Cells of Escherichia coli: Relationship with Cell Death. Appl. Environ. Microbiol. 70: 1545-1554 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»