This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haroune, N.
Right arrow Articles by Delort, A.-M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haroune, N.
Right arrow Articles by Delort, A.-M.
Agricola
Right arrow Articles by Haroune, N.
Right arrow Articles by Delort, A.-M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2002, p. 6114-6120, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6114-6120.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Benzothiazole Degradation by Rhodococcus pyridinovorans Strain PA: Evidence of a Catechol 1,2-Dioxygenase Activity

Nicolas Haroune,1 Bruno Combourieu,1 Pascale Besse,1 Martine Sancelme,1 Thorsten Reemtsma,2 Achim Kloepfer,2 Amer Diab,3 Jeremy S. Knapp,3 Simon Baumberg,4 and Anne-Marie Delort1*

Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 CNRS-Université Blaise Pascal, 63177 Aubière Cedex, France,1 Department of Water Quality Control, Technical University of Berlin, Sekr KF 4, 10623 Berlin, Germany,2 Division of Microbiology, School of Biochemistry and Molecular Biology,3 School of Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom4

Received 24 June 2002/ Accepted 9 September 2002

The pathway for biodegradation of benzothiazole (BT) and 2-hydroxybenzothiazole (OBT) by Rhodococcus pyridinovorans strain PA was studied in detail. The kinetics of biodegradation were monitored by in situ 1H nuclear magnetic resonance (NMR) in parallel with reversed-phase high-performance liquid chromatography (HPLC). Successive oxidations from BT to OBT and then from OBT to dihydroxybenzothiazole were observed. Further insight was obtained by using a mutant strain with impaired ability to grow on BT and OBT. The precise structure of another intermediate was determined by in situ two-dimensional 1H-13C NMR and HPLC-electrospray ionization mass spectrometry; this intermediate was found to be a ring-opening product (a diacid structure). Detection of this metabolite, together with the results obtained by 1H and 19F NMR when cells were incubated with 3-fluorocatechol, demonstrated that a catechol 1,2-dioxygenase is involved in a pathway for biodegradation of BTs in this Rhodococcus strain. Our results show that catechol 1,2-dioxygenase and catechol 2,3-dioxygenase activities may both be involved in the biodegradation of BTs depending on the culture conditions.

* Corresponding author. Mailing address: Laboratoire de Synthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504 CNRS-Université Blaise Pascal, 63177 Aubière Cedex, France. Phone: 33 4 73 40 77 14. Fax: 33 4 73 40 77 17. E-mail: amdelort{at}chimtp.univ-bpclermont.fr.

Applied and Environmental Microbiology, December 2002, p. 6114-6120, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6114-6120.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bunescu, A., Besse-Hoggan, P., Sancelme, M., Mailhot, G., Delort, A.-M. (2008). Fate of the Nitrilotriacetic Acid-Fe(III) Complex during Photodegradation and Biodegradation by Rhodococcus rhodochrous. Appl. Environ. Microbiol. 74: 6320-6326 [Abstract] [Full Text]  
  • Bunescu, A., Besse-Hoggan, P., Sancelme, M., Mailhot, G., Delort, A.-M. (2008). Comparison of Microbial and Photochemical Processes and Their Combination for Degradation of 2-Aminobenzothiazole. Appl. Environ. Microbiol. 74: 2976-2984 [Abstract] [Full Text]  
  • Uroz, S., Chhabra, S. R., Camara, M., Williams, P., Oger, P., Dessaux, Y. (2005). N-Acylhomoserine lactone quorum-sensing molecules are modified and degraded by Rhodococcus erythropolis W2 by both amidolytic and novel oxidoreductase activities. Microbiology 151: 3313-3322 [Abstract] [Full Text]  
  • Catallo, W. J., Junk, T. (2005). Transformation of Benzothiazole in Estuarine Sediments. J. Environ. Qual. 34: 1746-1754 [Abstract] [Full Text]  
  • Haroune, N., Combourieu, B., Besse, P., Sancelme, M., Kloepfer, A., Reemtsma, T., De Wever, H., Delort, A.-M. (2004). Metabolism of 2-Mercaptobenzothiazole by Rhodococcus rhodochrous. Appl. Environ. Microbiol. 70: 6315-6319 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»