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Applied and Environmental Microbiology, December 2002, p. 6138-6145, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6138-6145.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic Variability in Puccinia striiformis f. sp. tritici Populations Sampled on a Local Scale during Natural Epidemics

Lorys M. M. A. Villaréal,1 Christian Lannou,1 Claude de Vallavieille-Pope,1 and Claire Neema2*

Unité de Pathologie Végétale et Epidémiologie, INRA, 78850 Thiverval Grignon,1 Laboratoire de Pathologie Végétale, INRA, Institut National Agronomique Paris-Grignon, 75231 Paris Cedex 05, France2

Received 17 April 2002/ Accepted 11 September 2002

This study investigated genetic polymorphism on a local scale in Puccinia striiformis f. sp. tritici populations during natural epidemics, in four fields located in northern France and sampled in 1998 or 1999. Two hundred and forty-seven isolates were analyzed for their amplified fragment length polymorphism (AFLP) pattern through four primer combinations, and 194 of them were also tested for their virulence factors. Only one or two pathotypes were found in each field, and all isolates had virulence v17, matching the recently introduced Yr17 resistance gene. Polymorphism on a field scale was low. Although 67 loci were polymorphic, 77% of the isolates had the same AFLP pattern, all other patterns being rare or unique. Analyses of the genetic distance between AFLP patterns based on the Jaccard index allowed us to define 12 groups, but a bootstrap analysis showed that all isolates could be assigned to a single clonal lineage. This leads us to conclude that P. striiformis f. sp. tritici populations are clonal on a field scale in northern France.

* Corresponding author. Mailing address: Laboratoire de Pathologie Végétale, INRA, Institut National Agronomique Paris-Grignon, 16 rue Claude Bernard, 75231 Paris Cedex 05, France. Phone: 33 1 44081705. Fax: 33 1 44081631. E-mail: neema{at}inapg.inra.fr.

Applied and Environmental Microbiology, December 2002, p. 6138-6145, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6138-6145.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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