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Applied and Environmental Microbiology, December 2002, p. 6403-6404, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6403-6404.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

The Environmental Pathogen Mycobacterium ulcerans Grows in Amphibian Cells at Low Temperatures

Michel Drancourt,1 Vincent Jarlier,2 and Didier Raoult1*

Unité des Rickettsies CNRS UPRES A 6020, Faculté de Médecine, IFR 48, Université de la Méditerranée, 13385 Marseille,1 Laboratoire de Bactériologie, Faculté de Médecine Pitié-Salpétrière, Paris, France2

Received 26 July 2002/ Accepted 11 September 2002

Mycobacterium ulcerans, the etiological agent of Buruli ulcers, is an environmental pathogen. We cultivated it in an amphibian (XTC-2) cell line that grows at 28°C. By counting of Ziehl-Neelsen-stained mycobacteria and by quantitative PCR analysis, we found that M. ulcerans multiplies rapidly in association with XTC-2 cells. Transmission electron microscopy demonstrated the presence of intracellular M. ulcerans microorganisms. These data suggest an intracellular environmental niche, and we propose use of XTC-2 cells for isolation of M. ulcerans from environmental sources.

* Corresponding author. Mailing address: Unité des Rickettsies CNRS UPRES A 6020, Faculté de Médecine, IFR 48, Université de la Méditerranée, 27 Bd. Jean Moulin, 13385 Marseille, France. Phone: 33 (0)4 91 32 43 75. Fax: 33 (0)4 91 38 77 72. E-mail: didier.raoult{at}medecine.univ-mrs.fr.

Applied and Environmental Microbiology, December 2002, p. 6403-6404, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6403-6404.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Eddyani, M., Ofori-Adjei, D., Teugels, G., De Weirdt, D., Boakye, D., Meyers, W. M., Portaels, F. (2004). Potential Role for Fish in Transmission of Mycobacterium ulcerans Disease (Buruli Ulcer): an Environmental Study. Appl. Environ. Microbiol. 70: 5679-5681 [Abstract] [Full Text]  
  • Kotlowski, R., Martin, A., Ablordey, A., Chemlal, K., Fonteyne, P.-A., Portaels, F. (2004). One-tube cell lysis and DNA extraction procedure for PCR-based detection of Mycobacterium ulcerans in aquatic insects, molluscs and fish. J Med Microbiol 53: 927-933 [Abstract] [Full Text]  


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