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Applied and Environmental Microbiology, December 2002, p. 6425-6428, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6425-6428.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantitative Determination of Bile Salt Hydrolase Activity in Bacteria Isolated from the Small Intestine of Chickens

Ane Knarreborg,* Ricarda M. Engberg, Søren K. Jensen, and Bent B. Jensen

Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, DK-8830 Tjele, Denmark

Received 20 May 2002/ Accepted 6 September 2002

A quantitative assay based on high-performance liquid chromatography analysis of bile salts and bacterial protein determination was established for investigating bile salt hydrolase (BSH) activity in bacteria isolated from the small intestine of chickens. Bacteria were isolated using various media and were subsequently grouped according to cell morphology, fermentation profile, and 16S ribosomal DNA sequence. Representative isolates from each bacterial group were assayed for BSH activity. The isolates differed in BSH activity with respect to the state of growth and preculturing with and without taurochenodeoxycholate. The highest levels of BSH activity were found with Enterococcus faecium and Clostridium perfringens.

* Corresponding author. Mailing address: Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark. Phone: 4589991170. Fax: 4589991378. E-mail: ane.knarreborg{at}agrsci.dk.

Applied and Environmental Microbiology, December 2002, p. 6425-6428, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6425-6428.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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