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Applied and Environmental Microbiology, December 2002, p. 6446-6450, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6446-6450.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Phylogenetic Analysis of Acute Bee Paralysis Virus Strains

Tamás Bakonyi,1,{dagger} Elvira Grabensteiner,2,3 Jolanta Kolodziejek,2 Miklós Rusvai,1 Grazyna Topolska,4 Wolfgang Ritter,5 and Norbert Nowotny2,6,7*

Department of Microbiology and Infectious Diseases, Faculty of Veterinary Sciences, Szent István University, H-1143 Budapest, Hungary,1 Clinical Virology Group, Institute of Virology,2 Institute of Hydrobiology, Ichthyology and Apidology, University of Veterinary Medicine, A-1210 Vienna,6 Department of Microbiology and Electron Microscopy, Agricultural Inspection Service and Research Centre Vienna, Austrian Agency for Health and Food Safety and Federal Office for Food Safety, A-1226 Vienna, Austria,3 Department of Infectious Diseases, Microbiology & Parasitology, Faculty of Veterinary Medicine, Warsaw Agricultural University, PL-03-849 Warsaw, Poland,4 Department of Bee Pathology, CVUA-Animal Health, D-79108 Freiburg, Germany,5 Department of Medical Microbiology, Faculty of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates7

Received 12 July 2002/ Accepted 12 September 2002

Reverse transcription-PCR assays have been established for a quick, sensitive, and specific diagnosis of acute bee paralysis virus (ABPV), a common virus of the honeybee (Apis mellifera), directly from clinical samples. A 3,071-nucleotide fragment of the ABPV genome, which includes the entire capsid polyprotein gene, was amplified from Austrian, German, Polish, and Hungarian ABPV samples and sequenced, and the sequences were compared. The alignment of a smaller fragment with ABPV sequences from the United States and the United Kingdom revealed nucleotide identity rates between 89 and 96%, respectively. Phylogenetic trees which display the molecular relationship between the viruses of different geographic origin were constructed.

* Corresponding author. Mailing address: Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, Vienna, Veterinärplatz 1, A-1210 Vienna, Austria. Phone: 43 1 25077 2704. Fax: 43 1 25077 2790. E-mail: Norbert.Nowotny{at}vu-wien.ac.at.

{dagger} Present address: Clinical Virology Group, Institute of Virology, University of Veterinary Medicine, A-1210 Vienna, Austria.

Applied and Environmental Microbiology, December 2002, p. 6446-6450, Vol. 68, No. 12
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.12.6446-6450.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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