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 Previous Article

Applied and Environmental Microbiology, February 2002, p. 1010-1013, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.1010-1013.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Quantitation of DNA Sequences in Environmental PCR Products by a Multiplexed, Bead-Based Method

Alexander Spiro and Mary Lowe*

Physics Department, Loyola College in Maryland, Baltimore, Maryland 21210

Received 14 August 2001/ Accepted 29 November 2001

A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater.

* Corresponding author. Mailing address: Physics Department, Loyola College in Maryland, 4501 N. Charles St., Baltimore, MD 21210. Phone: (410) 617-2709. Fax: (410) 617-2646. E-mail: mlowe{at}loyola.edu.

Applied and Environmental Microbiology, February 2002, p. 1010-1013, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.1010-1013.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chandler, D. P., Jarrell, A. E. (2004). Automated Purification and Suspension Array Detection of 16S rRNA from Soil and Sediment Extracts by Using Tunable Surface Microparticles. Appl. Environ. Microbiol. 70: 2621-2631 [Abstract] [Full Text]  
  • Emrich, S. J., Lowe, M., Delcher, A. L. (2003). PROBEmer: a web-based software tool for selecting optimal DNA oligos. Nucleic Acids Res 31: 3746-3750 [Abstract] [Full Text]  


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