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Applied and Environmental Microbiology, February 2002, p. 942-946, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.942-946.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genetic and Immunochemical Characterization of Thiocyanate-Degrading Bacteria in Lake Water

Manabu Yamasaki,1 Yasuhiko Matsushita,2 Motonobu Namura,1 Hiroshi Nyunoya,2 and Yoko Katayama1*

Faculty of Agriculture,1 Gene Research Center, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan2

Received 19 July 2001/ Accepted 2 November 2001

Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from activated sludge.

* Corresponding author. Mailing address: Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan. Phone: 81-42-367-5732. Fax: 81-42-367-5732. E-mail: katayama{at}cc.tuat.ac.jp.

Applied and Environmental Microbiology, February 2002, p. 942-946, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.942-946.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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