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Applied and Environmental Microbiology, February 2002, p. 947-951, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.947-951.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Two Forms of NAD-Dependent D-Mandelate Dehydrogenase in Enterococcus faecalis IAM 10071

Yusuke Tamura, Atsuko Ohkubo, Saho Iwai, Yoichiro Wada, Takeshi Shinoda, Kazuhito Arai, Shigeru Mineki, Mitsugi Iida, and Hayao Taguchi*

Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan

Received 21 June 2001/ Accepted 22 October 2001

Two forms of NAD-dependent D-mandelate dehydrogenase (D-ManDHs) were purified from Enterococcus faecalis IAM 10071. While these two enzymes consistently exhibited high activity toward large 2-ketoacid substrates that were branched at the C3 or C4 position, they gave distinctly different Km and Vmax values for these substrates and had distinct molecular weights by gel electrophoresis and gel filtration.

* Corresponding author. Mailing address: Department of Applied Biological Science, Faculty of Science and Technology, Science University of Tokyo, Noda, Chiba 278-8510, Japan. Phone: 0471-24-1501, ext. 3407. Fax: 0471-23-9767. E-mail: htaguchi{at}rs.noda.sut.ac.jp.

Applied and Environmental Microbiology, February 2002, p. 947-951, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.947-951.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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