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Applied and Environmental Microbiology, February 2002, p. 989-994, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.989-994.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Detection and Quantification of Pfiesteria piscicida by Using the Mitochondrial Cytochrome b Gene{dagger}

Huan Zhang and Senjie Lin*

Department of Marine Sciences, University of Connecticut, Groton, Connecticut 06340

Received 24 August 2001/ Accepted 13 November 2001

Mitochondrial cytochrome b was isolated from the dinoflagellate Pfiesteria piscicida, and the utility of the gene for species identification was examined. One of the primer sets designed was shown to be highly specific for P. piscicida. A time step PCR protocol was used to demonstrate the potential of this primer set for quantification of this species.

* Corresponding author. Mailing address: Department of Marine Sciences, University of Connecticut, Groton, CT 06340. Phone: (860) 405-9168. Fax: (860) 405-9153. E-mail: senjie.lin{at}uconn.edu.

{dagger} ECOHAB publication number 34.

Applied and Environmental Microbiology, February 2002, p. 989-994, Vol. 68, No. 2
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.2.989-994.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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