This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gouesbet, G.
Right arrow Articles by Boyaval, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gouesbet, G.
Right arrow Articles by Boyaval, P.
Agricola
Right arrow Articles by Gouesbet, G.
Right arrow Articles by Boyaval, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2002, p. 1055-1063, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1055-1063.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Two-Dimensional Electrophoresis Study of Lactobacillus delbrueckii subsp. bulgaricus Thermotolerance

Gwenola Gouesbet,1,2 Gwenael Jan,1 and Patrick Boyaval1*

Laboratoire de Recherches de Technologie Laitière, INRA, 35042 Rennes Cedex,1 Rhodia-Food, Z. A. de Buxières, 86220 Dangé St. Romain, France2

Received 6 September 2001/ Accepted 26 November 2001

The response of Lactobacillus delbrueckii subsp. bulgaricus cells to heat stress was studied by use of a chemically defined medium. Two-dimensional electrophoresis (2-DE) analysis was used to correlate the kinetics of heat shock protein (HSP) induction with cell recovery from heat injury. We demonstrated that enhanced viability, observed after 10 min at 65°C, resulted from the overexpression of HSP and from mechanisms not linked to protein synthesis. In order to analyze the thermoadaptation mechanisms involved, thermoresistant variants were selected. These variants showed enhanced constitutive tolerance toward heat shock. However, contrary to the wild-type strain, these variants were poorly protected after osmotic or heat pretreatments. This result suggests that above a certain threshold, cells reach a maximum level of protection that cannot be easily exceeded. A comparison of protein patterns showed that the variants were able to induce more rapidly their adaptive mechanisms than the original strain. In particular, the variants were able to express constitutively more HSP, leading to the higher level of thermoprotection observed. This is the first report of the study by 2-DE of the heat stress response in L. delbrueckii subsp. bulgaricus.

* Corresponding author. Mailing address: INRA, Laboratoire de Recherches de Technologie Laitière, 65 rue de St. Brieuc, 35042 Rennes Cedex, France. Phone: 33-(0) 2 23 48 53 39. Fax: 33-(0) 2 23 48 53 50. E-mail: boyaval{at}rennes.inra.fr.

Applied and Environmental Microbiology, March 2002, p. 1055-1063, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1055-1063.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gagnaire, V., Jardin, J., Jan, G., Lortal, S. (2009). Invited review: Proteomics of milk and bacteria used in fermented dairy products: From qualitative to quantitative advances. J DAIRY SCI 92: 811-825 [Abstract] [Full Text]  
  • Santarelli, M., Gatti, M., Lazzi, C., Bernini, V., Zapparoli, G. A., Neviani, E. (2008). Whey Starter for Grana Padano Cheese: Effect of Technological Parameters on Viability and Composition of the Microbial Community. J DAIRY SCI 91: 883-891 [Abstract] [Full Text]  
  • Sanchez, B., Champomier-Verges, M.-C., Anglade, P., Baraige, F., de los Reyes-Gavilan, C. G., Margolles, A., Zagorec, M. (2005). Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809. J. Bacteriol. 187: 5799-5808 [Abstract] [Full Text]  
  • De Angelis, M., Di Cagno, R., Huet, C., Crecchio, C., Fox, P. F., Gobbetti, M. (2004). Heat Shock Response in Lactobacillus plantarum. Appl. Environ. Microbiol. 70: 1336-1346 [Abstract] [Full Text]  
  • Prasad, J., McJarrow, P., Gopal, P. (2003). Heat and Osmotic Stress Responses of Probiotic Lactobacillus rhamnosus HN001 (DR20) in Relation to Viability after Drying. Appl. Environ. Microbiol. 69: 917-925 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»