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Applied and Environmental Microbiology, March 2002, p. 1228-1231, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1228-1231.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Cyt2Bc Toxin from Bacillus thuringiensis subsp. medellin

Victor Juárez-Pérez,1 Alejandra Guerchicoff,2 Clara Rubinstein,2 and Armelle Delécluse1*

Laboratoire des Bactéries et Champignons Entomopathogènes, Institut Pasteur, 75724 Paris Cedex 15, France,1 Facultad de Ciencias Exactas y Naturales, Departamento de Qu|$$|Aa|fimica Biológica, Ciudad Universitaria, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina2

Received 29 August 2001/ Accepted 2 January 2002

We cloned and sequenced a new cytolysin gene from Bacillus thuringiensis subsp. medellin. Three IS240-like insertion sequence elements and the previously cloned cyt1Ab and p21 genes were found in the vicinity of the cytolysin gene. The cytolysin gene encodes a protein 29.7 kDa in size that is 91.5% identical to Cyt2Ba from Bacillus thuringiensis subsp. israelensis and has been designated Cyt2Bc. Inclusions containing Cyt2Bc were purified from the crystal-negative strain SPL407 of B. thuringiensis. Cyt2Bc reacted weakly with antibodies directed against Cyt2Ba and was not recognized by an antiserum directed against the reference cytolysin Cyt1Aa. Cyt2Bc was hemolytic only upon activation with trypsin and had only one-third to one-fifth of the activity of Cyt2Ba, depending on the activation time. Cyt2Bc was also mosquitocidal against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus, including strains resistant to the Bacillus sphaericus binary toxin. Its toxicity was half of that of Cyt2Ba on all mosquito species except resistant C. quinquefasciatus.

* Corresponding author. Mailing address: Laboratoire des Bactéries et Champignons Entomopathogènes, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 40 61 31 80. Fax: (33) 1 40 61 30 44. E-mail: armdel{at}pasteur.fr.

Applied and Environmental Microbiology, March 2002, p. 1228-1231, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1228-1231.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Manasherob, R., Itsko, M., Sela-Baranes, N., Ben-Dov, E., Berry, C., Cohen, S., Zaritsky, A. (2006). Cyt1Ca from Bacillus thuringiensis subsp. israelensis: production in Escherichia coli and comparison of its biological activities with those of other Cyt-like proteins.. Microbiology 152: 2651-2659 [Abstract] [Full Text]  


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