This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lu, Y.
Right arrow Articles by Goodman, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lu, Y.
Right arrow Articles by Goodman, K.
Agricola
Right arrow Articles by Lu, Y.
Right arrow Articles by Goodman, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2002, p. 1436-1439, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1436-1439.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Isolation and Genotyping of Helicobacter pylori from Untreated Municipal Wastewater

Yingzhi Lu,1 Thomas E. Redlinger,1* Raquel Avitia,1 Adriana Galindo,1 and Karen Goodman2

Department of Biological Sciences, University of Texas, El Paso, Texas 79968,1 Houston School of Public Health, University of Texas, Houston, Texas 779022

Received 10 October 2001/ Accepted 17 December 2001

For this study, we isolated Helicobacter pylori from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative H. pylori isolates were verified by PCR. Eleven H. pylori isolates were genotyped and fell into four vacA classes: those with the vacA allelic variants s1a and m1, s1b and m1, s2 and m2, or s2 and m1. Most H. pylori isolates were of the vacA s1a/m1 type, which has been shown to be associated with advanced diseases based on genotyping of H. pylori from gastric cancer patients. These results demonstrated that H. pylori survives in water and may be a potential source of H. pylori transmission, especially where water is not adequately treated.

* Corresponding author. Mailing address: Department of Biological Sciences, The University of Texas, El Paso, TX 79968. Phone: (915) 747-6995. Fax: (915) 747-5808. E-mail: thomasr{at}utep.edu.

Applied and Environmental Microbiology, March 2002, p. 1436-1439, Vol. 68, No. 3
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.3.1436-1439.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Janzon, A., Sjoling, A., Lothigius, A., Ahmed, D., Qadri, F., Svennerholm, A.-M. (2009). Failure To Detect Helicobacter pylori DNA in Drinking and Environmental Water in Dhaka, Bangladesh, Using Highly Sensitive Real-Time PCR Assays. Appl. Environ. Microbiol. 75: 3039-3044 [Abstract] [Full Text]  
  • Sen, K., Schable, N. A., Lye, D. J. (2007). Development of an Internal Control for Evaluation and Standardization of a Quantitative PCR Assay for Detection of Helicobacter pylori in Drinking Water. Appl. Environ. Microbiol. 73: 7380-7387 [Abstract] [Full Text]  
  • Solnick, J. V., Fong, J., Hansen, L. M., Chang, K., Canfield, D. R., Parsonnet, J. (2006). Acquisition of Helicobacter pylori Infection in Rhesus Macaques Is Most Consistent with Oral-Oral Transmission.. J. Clin. Microbiol. 44: 3799-3803 [Abstract] [Full Text]  
  • Kusters, J. G., van Vliet, A. H. M., Kuipers, E. J. (2006). Pathogenesis of Helicobacter pylori Infection. Clin. Microbiol. Rev. 19: 449-490 [Abstract] [Full Text]  
  • Shahamat, M., Alavi, M., Watts, J. E. M., Gonzalez, J. M., Sowers, K. R., Maeder, D. W., Robb, F. T. (2004). Development of Two PCR-Based Techniques for Detecting Helical and Coccoid Forms of Helicobacter pylori. J. Clin. Microbiol. 42: 3613-3619 [Abstract] [Full Text]  
  • Cole, S. P., Harwood, J., Lee, R., She, R., Guiney, D. G. (2004). Characterization of Monospecies Biofilm Formation by Helicobacter pylori. J. Bacteriol. 186: 3124-3132 [Abstract] [Full Text]  
  • Adams, B. L., Bates, T. C., Oliver, J. D. (2003). Survival of Helicobacter pylori in a Natural Freshwater Environment. Appl. Environ. Microbiol. 69: 7462-7466 [Abstract] [Full Text]  
  • Datta, S., Khan, A., Nandy, R. K., Rehman, M., Sinha, S., Chattopadhyay, S., Das, S. C., Nair, G. B. (2003). Environmental Isolates of Aeromonas spp. Harboring the cagA-Like Gene of Helicobacter pylori. Appl. Environ. Microbiol. 69: 4291-4295 [Abstract] [Full Text]  
  • Degnan, A. J., Sonzogni, W. C., Standridge, J. H. (2003). Development of a Plating Medium for Selection of Helicobacter pylori from Water Samples. Appl. Environ. Microbiol. 69: 2914-2918 [Abstract] [Full Text]  
  • Figueroa, G., Faundez, G., Troncoso, M., Navarrete, P., Toledo, M. S. (2002). Immunoglobulin G Antibody Response to Infection with Coccoid Forms of Helicobacter pylori. CVI 9: 1067-1071 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»