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Applied and Environmental Microbiology, April 2002, p. 1610-1615, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1610-1615.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Determination of Subunit Composition of Clostridium cellulovorans Cellulosomes That Degrade Plant Cell Walls

Koichiro Murashima, Akihiko Kosugi, and Roy H. Doi*

Sections of Molecular and Cellular Biology, University of California, Davis, California 95616

Received 8 August 2001/ Accepted 15 January 2002

Clostridium cellulovorans produces a cellulase enzyme complex (cellulosome). In this study, we isolated two plant cell wall-degrading cellulosomal fractions from culture supernatant of C. cellulovorans and determined their subunit compositions and enzymatic activities. One of the cellulosomal fractions showed fourfold-higher plant cell wall-degrading activity than the other. Both cellulosomal fractions contained the same nine subunits (the scaffolding protein CbpA, endoglucanases EngE and EngK, cellobiohydrolase ExgS, xylanase XynA, mannanase ManA, and three unknown proteins), although the relative amounts of the subunits differed. Since only cellobiose was released from plant cell walls by the cellulosomal fractions, cellobiohydrolases were considered to be key enzymes for plant cell wall degradation.

* Corresponding author. Mailing address: Sections of Molecular and Cellular Biology, University of California, Davis, CA 95616. Phone: (530) 752-3191. Fax: (530) 752-3085. E-mail: rhdoi{at}ucdavis.edu.

Applied and Environmental Microbiology, April 2002, p. 1610-1615, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1610-1615.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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