This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Arias, C. R. Articles by Parish, M. E. Search for Related Content PubMed PubMed Citation Articles by Arias, C. R. Articles by Parish, M. E. Agricola Articles by Arias, C. R. Articles by Parish, M. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2002, p. 1955-1961, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.1955-1961.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Yeast Species Associated with Orange Juice: Evaluation of Different Identification Methods

Citrus Research and Education Center, University of Florida, IFAS, Lake Alfred, Florida 33850

Received 1 October 2001/ Accepted 14 January 2002

Five different methods were used to identify yeast isolates from a variety of citrus juice sources. A total of 99 strains, including reference strains, were identified using a partial sequence of the 26S rRNA gene, restriction pattern analysis of the internal transcribed spacer region (5.8S-ITS), classical methodology, the RapID Yeast Plus system, and API 20C AUX. Twenty-three different species were identified representing 11 different genera. Distribution of the species was considerably different depending on the type of sample. Fourteen different species were identified from pasteurized single-strength orange juice that had been contaminated after pasteurization (PSOJ), while only six species were isolated from fresh-squeezed, unpasteurized orange juice (FSOJ). Among PSOJ isolates, Candida intermedia and Candida parapsilosis were the predominant species. Hanseniaspora occidentalis and Hanseniaspora uvarum represented up to 73% of total FSOJ isolates. Partial sequence of the 26S rRNA gene yielded the best results in terms of correct identification, followed by classical techniques and 5.8S-ITS analysis. The commercial identification kits RapID Yeast Plus system and API 20C AUX were able to correctly identify only 35 and 13% of the isolates, respectively. Six new 5.8S-ITS profiles were described, corresponding to Clavispora lusitaniae, Geotrichum citri-aurantii, H. occidentalis, H. vineae, Pichia fermentans, and Saccharomycopsis crataegensis. With the addition of these new profiles to the existing database, the use of 5.8S-ITS sequence became the best tool for rapid and accurate identification of yeast isolates from orange juice.

This study was approved for publication as Journal Series no. R-08477 of the Florida Agricultural Experiment Station.

This article has been cited by other articles:

• Wenning, M., Seiler, H., Scherer, S. (2002). Fourier-Transform Infrared Microspectroscopy, a Novel and Rapid Tool for Identification of Yeasts. Appl. Environ. Microbiol. 68: 4717-4721 [Abstract] [Full Text]