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Applied and Environmental Microbiology, April 2002, p. 2066-2070, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2066-2070.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Effects of pH and Magnetic Material on Immunomagnetic Separation of Cryptosporidium Oocysts from Concentrated Water Samples

Ryan C. Kuhn, Channah M. Rock, and Kevin H. Oshima*

Department of Biology, New Mexico State University, Las Cruces, New Mexico 88003

Received 15 October 2001/ Accepted 25 January 2002

In this study, we examined the effect that magnetic materials and pH have on the recoveries of Cryptosporidium oocysts by immunomagnetic separation (IMS). We determined that particles that were concentrated on a magnet during bead separation have no influence on oocyst recovery; however, removal of these particles did influence pH values. The optimal pH of the IMS was determined to be 7.0. The numbers of oocysts recovered from deionized water at pH 7.0 were 26.3% higher than those recovered from samples that were not at optimal pH. The results indicate that the buffers in the IMS kit did not adequately maintain an optimum pH in some water samples. By adjusting the pH of concentrated environmental water samples to 7.0, recoveries of oocysts increased by 26.4% compared to recoveries from samples where the pH was not adjusted.

* Corresponding author. Mailing address: Department of Biology, New Mexico State University, Dept. 3AF, P.O. Box 30001, Las Cruces, NM 88003. Phone: (505) 646-2092. Fax: (505) 646-5665. E-mail: koshima{at}nmsu.edu.

Applied and Environmental Microbiology, April 2002, p. 2066-2070, Vol. 68, No. 4
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.4.2066-2070.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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