This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frias-Lopez, J.
Right arrow Articles by Fouke, B. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frias-Lopez, J.
Right arrow Articles by Fouke, B. W.
Agricola
Right arrow Articles by Frias-Lopez, J.
Right arrow Articles by Fouke, B. W.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2002, p. 2214-2228, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2214-2228.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Partitioning of Bacterial Communities between Seawater and Healthy, Black Band Diseased, and Dead Coral Surfaces

Jorge Frias-Lopez, Aubrey L. Zerkle, George T. Bonheyo, and Bruce W. Fouke*

Department of Geology, University of Illinois, Urbana, Illinois 61801

Received 21 December 2001/ Accepted 25 February 2002

Distinct partitioning has been observed in the composition and diversity of bacterial communities inhabiting the surface and overlying seawater of three coral species infected with black band disease (BBD) on the southern Caribbean island of Curaçao, Netherlands Antilles. PCR amplification and sequencing of bacterial 16S rRNA genes (rDNA) with universally conserved primers have identified over 524 unique bacterial sequences affiliated with 12 bacterial divisions. The molecular sequences exhibited less than 5% similarity in bacterial community composition between seawater and the healthy, black band diseased, and dead coral surfaces. The BBD bacterial mat rapidly migrates across and kills the coral tissue. Clone libraries constructed from the BBD mat were comprised of eight bacterial divisions and 13% unknowns. Several sequences representing bacteria previously found in other marine and terrestrial organisms (including humans) were isolated from the infected coral surfaces, including Clostridium spp., Arcobacter spp., Campylobacter spp., Cytophaga fermentans, Cytophaga columnaris, and Trichodesmium tenue.

* Corresponding author. Mailing address: Department of Geology, University of Illinois, 1301 W. Green St., Urbana, IL 61801. Phone: (217) 244-5431. Fax: (217) 244-4996. E-mail: fouke{at}uiuc.edu.

Applied and Environmental Microbiology, May 2002, p. 2214-2228, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2214-2228.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sato, Y., Bourne, D. G., Willis, B. L. (2009). Dynamics of seasonal outbreaks of black band disease in an assemblage of Montipora species at Pelorus Island (Great Barrier Reef, Australia). Proc R Soc B 276: 2795-2803 [Abstract] [Full Text]  
  • Davis, J. P., Youssef, N. H., Elshahed, M. S. (2009). Assessment of the Diversity, Abundance, and Ecological Distribution of Members of Candidate Division SR1 Reveals a High Level of Phylogenetic Diversity but Limited Morphotypic Diversity. Appl. Environ. Microbiol. 75: 4139-4148 [Abstract] [Full Text]  
  • Raina, J.-B., Tapiolas, D., Willis, B. L., Bourne, D. G. (2009). Coral-Associated Bacteria and Their Role in the Biogeochemical Cycling of Sulfur. Appl. Environ. Microbiol. 75: 3492-3501 [Abstract] [Full Text]  
  • Kellogg, C. A., Lisle, J. T., Galkiewicz, J. P. (2009). Culture-Independent Characterization of Bacterial Communities Associated with the Cold-Water Coral Lophelia pertusa in the Northeastern Gulf of Mexico. Appl. Environ. Microbiol. 75: 2294-2303 [Abstract] [Full Text]  
  • Sekar, R., Kaczmarsky, L. T., Richardson, L. L. (2009). Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals. Appl. Environ. Microbiol. 75: 2581-2584 [Abstract] [Full Text]  
  • Zeevi Ben Yosef, D., Ben-Dov, E., Kushmaro, A. (2008). Amorphus coralli gen. nov., sp. nov., a marine bacterium isolated from coral mucus, belonging to the order Rhizobiales. Int. J. Syst. Evol. Microbiol. 58: 2704-2709 [Abstract] [Full Text]  
  • Vega Thurber, R. L., Barott, K. L., Hall, D., Liu, H., Rodriguez-Mueller, B., Desnues, C., Edwards, R. A., Haynes, M., Angly, F. E., Wegley, L., Rohwer, F. L. (2008). Metagenomic analysis indicates that stressors induce production of herpes-like viruses in the coral Porites compressa. Proc. Natl. Acad. Sci. USA 105: 18413-18418 [Abstract] [Full Text]  
  • Bourne, D. G., Boyett, H. V., Henderson, M. E., Muirhead, A., Willis, B. L. (2008). Identification of a Ciliate (Oligohymenophorea: Scuticociliatia) Associated with Brown Band Disease on Corals of the Great Barrier Reef. Appl. Environ. Microbiol. 74: 883-888 [Abstract] [Full Text]  
  • Beman, J.M., Roberts, K. J., Wegley, L., Rohwer, F., Francis, C. A. (2007). Distribution and Diversity of Archaeal Ammonia Monooxygenase Genes Associated with Corals. Appl. Environ. Microbiol. 73: 5642-5647 [Abstract] [Full Text]  
  • Myers, J. L., Sekar, R., Richardson, L. L. (2007). Molecular Detection and Ecological Significance of the Cyanobacterial Genera Geitlerinema and Leptolyngbya in Black Band Disease of Corals. Appl. Environ. Microbiol. 73: 5173-5182 [Abstract] [Full Text]  
  • Ulstrup, K. E., Kuhl, M., Bourne, D. G. (2007). Zooxanthellae Harvested by Ciliates Associated with Brown Band Syndrome of Corals Remain Photosynthetically Competent. Appl. Environ. Microbiol. 73: 1968-1975 [Abstract] [Full Text]  
  • Ainsworth, T. D., Kramasky-Winter, E., Loya, Y., Hoegh-Guldberg, O., Fine, M. (2007). Coral Disease Diagnostics: What's between a Plague and a Band?. Appl. Environ. Microbiol. 73: 981-992 [Abstract] [Full Text]  
  • Sekar, R., Mills, D. K., Remily, E. R., Voss, J. D., Richardson, L. L. (2006). Microbial Communities in the Surface Mucopolysaccharide Layer and the Black Band Microbial Mat of Black Band-Diseased Siderastrea siderea. Appl. Environ. Microbiol. 72: 5963-5973 [Abstract] [Full Text]  
  • Ainsworth, T. D., Fine, M., Blackall, L. L., Hoegh-Guldberg, O. (2006). Fluorescence In Situ Hybridization and Spectral Imaging of Coral-Associated Bacterial Communities. Appl. Environ. Microbiol. 72: 3016-3020 [Abstract] [Full Text]  
  • Buchan, A., Gonzalez, J. M., Moran, M. A. (2005). Overview of the Marine Roseobacter Lineage. Appl. Environ. Microbiol. 71: 5665-5677 [Full Text]  
  • Cervino, J. M., Hayes, R. L., Polson, S. W., Polson, S. C., Goreau, T. J., Martinez, R. J., Smith, G. W. (2004). Relationship of Vibrio Species Infection and Elevated Temperatures to Yellow Blotch/Band Disease in Caribbean Corals. Appl. Environ. Microbiol. 70: 6855-6864 [Abstract] [Full Text]  
  • Frias-Lopez, J., Klaus, J. S., Bonheyo, G. T., Fouke, B. W. (2004). Bacterial Community Associated with Black Band Disease in Corals. Appl. Environ. Microbiol. 70: 5955-5962 [Abstract] [Full Text]  
  • Frias-Lopez, J., Bonheyo, G. T., Fouke, B. W. (2004). Identification of Differential Gene Expression in Bacteria Associated with Coral Black Band Disease by Using RNA-Arbitrarily Primed PCR. Appl. Environ. Microbiol. 70: 3687-3694 [Abstract] [Full Text]  
  • PIDIYAR, V. J., JANGID, K., PATOLE, M. S., SHOUCHE, Y. S. (2004). STUDIES ON CULTURED AND UNCULTURED MICROBIOTA OF WILD CULEX QUINQUEFASCIATUS MOSQUITO MIDGUT BASED ON 16S RIBOSOMAL RNA GENE ANALYSIS. Am J Trop Med Hyg 70: 597-603 [Abstract] [Full Text]  
  • Frias-Lopez, J., Bonheyo, G. T., Jin, Q., Fouke, B. W. (2003). Cyanobacteria Associated with Coral Black Band Disease in Caribbean and Indo-Pacific Reefs. Appl. Environ. Microbiol. 69: 2409-2413 [Abstract] [Full Text]  
  • Zengler, K., Toledo, G., Rappe, M., Elkins, J., Mathur, E. J., Short, J. M., Keller, M. (2002). Cultivating the uncultured. Proc. Natl. Acad. Sci. USA 99: 15681-15686 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»