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Applied and Environmental Microbiology, May 2002, p. 2550-2554, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2550-2554.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biochemical and Genetic Characterization of the Two-Peptide Bacteriocin Enterocin 1071 Produced by Enterococcus faecalis FAIR-E 309

Charles M. A. P. Franz,1 Alexandra Grube,1 Anette Herrmann,1 Hikmate Abriouel,1 Joachim Stärke,2 Angiolella Lombardi,3 Bernhard Tauscher,2 and Wilhelm H. Holzapfel1*

Institute for Biotechnology and Molecular Biology,1 Institute for Chemistry and Biology, Federal Research Centre for Nutrition, D-76131 Karlsruhe, Germany,2 Veneto Agricoltura, Istituto per la Qualità e le Tecnologie Agroalimentari, 36016 Thiene (VI), Italy3

Received 20 August 2001/ Accepted 30 November 2001

The structural genes for the two-peptide bacteriocin enterocin 1071 (Ent1071) in Enterococcus faecalis FAIR-E 309 were cloned. DNA sequence analysis showed that the enterocin 1071A (Ent1071A) peptide of strain FAIR-E 309 differed by two amino acids from the Ent1071A reported for E. faecalis BFE 1071 (E. Balla, L. M. T. Dicks, M. Du Toit, M. J. van der Merwe, and W. H. Holzapfel, Appl. Environ. Microbiol. 66:1298-1304, 2000), while the Ent1071B gene encoded identical peptides in these strains. However, resequencing of ent1071A from E. faecalis BFE 1071 showed that the Ent1071A peptide sequence reported previously was incorrect in two amino acids. Also, ent1071B in E. faecalis FAIR-E 309 encoded a prepeptide that was three amino acids shorter than that previously reported for E. faecalis BFE 1071 Ent1071B. A presumptive immunity gene (eni1071) was located downstream of the bacteriocin structural genes. This gene was cloned into the heterologous host E. faecalis ATCC 19433 and was shown to confer immunity. A truncated ABC transporter gene was located upstream of the Ent1071 structural genes.

* Corresponding author. Mailing address: Institute for Biotechnology and Molecular Biology, Federal Research Centre for Nutrition, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany. Phone: 49-721-6625 450. Fax: 49-721-6625 453. E-mail: wilhelm.holzapfel{at}bfe.uni-karlsruhe.de.

Applied and Environmental Microbiology, May 2002, p. 2550-2554, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2550-2554.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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