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Applied and Environmental Microbiology, May 2002, p. 2614-2618, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2614-2618.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Formation of Protoplasts from Cultured Tobacco Cells and Arabidopsis thaliana by the Action of Cellulosomes and Pectate Lyase from Clostridium cellulovorans

Yutaka Tamaru,1,{dagger} Sadaharu Ui,1,{ddagger} Koichiro Murashima,1 Akihiko Kosugi,1 Helen Chan,1 Roy H. Doi,1* and Bo Liu2

Sections of Molecular and Cellular Biology,1 Plant Biology, University of California, Davis, California 956162

Received 15 October 2001/ Accepted 2 March 2002

The crude culture supernatants from Clostridium cellulovorans were tested for their ability to convert plant cells to protoplasts. The supernatants readily released protoplasts from cultured tobacco cells and Arabidopsis thaliana. The crude culture supernatant from pectin-grown cells was more active than supernatants from glucose-, cellobiose-, xylan-, and locust bean gum-grown cells. After removal of cellulosomes, the crude culture supernatant lost its protoplast formation activity. The protoplast formation activity of the crude culture supernatant from C. cellulovorans was more effective than those of commercial enzymes based on protein content.

* Corresponding author. Mailing address: Sections of Molecular and Cellular Biology, University of California, Davis, CA 95616. Phone: (530) 752-3191. Fax: (530) 752-3085. E-mail: rhdoi{at}ucdavis.edu.

{dagger} Present address: Faculty of Bioresources, Mie University, Tsu 514-8507, Japan.

{ddagger} Present address: Faculty of Engineering, Yamanashi University, Kofu 400-8511, Japan.

Applied and Environmental Microbiology, May 2002, p. 2614-2618, Vol. 68, No. 5
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.5.2614-2618.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Demain, A. L., Newcomb, M., Wu, J. H. D. (2005). Cellulase, Clostridia, and Ethanol. Microbiol. Mol. Biol. Rev. 69: 124-154 [Abstract] [Full Text]  
  • Han, S. O., Cho, H.-Y., Yukawa, H., Inui, M., Doi, R. H. (2004). Regulation of Expression of Cellulosomes and Noncellulosomal (Hemi)Cellulolytic Enzymes in Clostridium cellulovorans during Growth on Different Carbon Sources. J. Bacteriol. 186: 4218-4227 [Abstract] [Full Text]  
  • Murashima, K., Kosugi, A., Doi, R. H. (2003). Synergistic Effects of Cellulosomal Xylanase and Cellulases from Clostridium cellulovorans on Plant Cell Wall Degradation. J. Bacteriol. 185: 1518-1524 [Abstract] [Full Text]  
  • Kosugi, A., Murashima, K., Doi, R. H. (2002). Characterization of Two Noncellulosomal Subunits, ArfA and BgaA, from Clostridium cellulovorans That Cooperate with the Cellulosome in Plant Cell Wall Degradation. J. Bacteriol. 184: 6859-6865 [Abstract] [Full Text]  


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