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Applied and Environmental Microbiology, June 2002, p. 2726-2730, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2726-2730.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Biodegradation of cis-Dichloroethene as the Sole Carbon Source by a ß-Proteobacterium

Nicholas V. Coleman,1 Timothy E. Mattes,2 James M. Gossett,2 and Jim C. Spain1*

Air Force Research Laboratory-MLQL, Tyndall AFB, Florida 32403,1 School of Civil and Environmental Engineering, Cornell University, Ithaca, New York 148532

Received 10 December 2001/ Accepted 18 March 2002

An aerobic bacterium capable of growth on cis-dichloroethene (cDCE) as a sole carbon and energy source was isolated by enrichment culture. The 16S ribosomal DNA sequence of the isolate (strain JS666) had 97.9% identity to the sequence from Polaromonas vacuolata, indicating that the isolate was a ß-proteobacterium. At 20°C, strain JS666 grew on cDCE with a minimum doubling time of 73 ± 7 h and a growth yield of 6.1 g of protein/mol of cDCE. Chloride analysis indicated that complete dechlorination of cDCE occurred during growth. The half-velocity constant for cDCE transformation was 1.6 ± 0.2 µM, and the maximum specific substrate utilization rate ranged from 12.6 to 16.8 nmol/min/mg of protein. Resting cells grown on cDCE could transform cDCE, ethene, vinyl chloride, trans-dichloroethene, trichloroethene, and 1,2-dichloroethane. Epoxyethane was produced from ethene by cDCE-grown cells, suggesting that an epoxidation reaction is the first step in cDCE degradation.

* Corresponding author. Mailing address: Air Force Research Laboratory-MLQL Building 1117, 139 Barnes Dr., Tyndall AFB, FL 32403. Phone: (850) 283-6058. Fax: (850) 283-6090. E-mail: Jim.Spain{at}tyndall.af.mil.

Applied and Environmental Microbiology, June 2002, p. 2726-2730, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2726-2730.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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