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Applied and Environmental Microbiology, June 2002, p. 2731-2736, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2731-2736.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Identification of Family 38 {alpha}-Mannosidase of Bacillus sp. Strain GL1, Responsible for Complete Depolymerization of Xanthan

Hirokazu Nankai, Wataru Hashimoto, and Kousaku Murata*

Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan

Received 5 November 2001/ Accepted 12 March 2002

When cells of Bacillus sp. strain GL1 were grown in a medium containing xanthan as a carbon source, {alpha}-mannosidase exhibiting activity toward p-nitrophenyl-{alpha}-D-mannopyranoside (pNP-{alpha}-D-Man) was produced intracellularly. The 350-kDa {alpha}-mannosidase purified from a cell extract of the bacterium was a trimer comprising three identical subunits, each with a molecular mass of 110 kDa. The enzyme hydrolyzed pNP-{alpha}-D-Man (Km = 0.49 mM) and D-mannosyl-({alpha}-1,3)-D-glucose most efficiently at pH 7.5 to 9.0, indicating that the enzyme catalyzes the last step of the xanthan depolymerization pathway of Bacillus sp. strain GL1. The gene for {alpha}-mannosidase cloned most by using N-terminal amino acid sequence information contained an open reading frame (3,144 bp) capable of coding for a polypeptide with a molecular weight of 119,239. The deduced amino acid sequence showed homology with the amino acid sequences of {alpha}-mannosidases belonging to glycoside hydrolase family 38.

* Corresponding author. Mailing address: Department of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. Phone: 81-774-38-3766. Fax: 81-774-38-3767. E-mail: murata{at}food2.food.kyoto-u.ac.jp.

Applied and Environmental Microbiology, June 2002, p. 2731-2736, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2731-2736.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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