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Applied and Environmental Microbiology, June 2002, p. 2763-2769, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2763-2769.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Involvement of Rhodocyclus-Related Organisms in Phosphorus Removal in Full-Scale Wastewater Treatment Plants

Julie L. Zilles, Jordan Peccia,{dagger} Myeong-Woon Kim,{ddagger} Chun-Hsiung Hung,§ and Daniel R. Noguera*

Department of Civil and Environmental Engineering, University of Wisconsin—Madison, Madison, Wisconsin 53706

Received 18 December 2001/ Accepted 13 March 2002

The participation of organisms related to Rhodocyclus in full-scale enhanced biological phosphorus removal (EBPR) was investigated. By using fluorescent in situ hybridization techniques, the communities of Rhodocyclus-related organisms in two full-scale wastewater treatment plants were estimated to represent between 13 and 18% of the total bacterial population. However, the fractions of these communities that participated in polyphosphate accumulation depended on the type of treatment process evaluated. In a University of Cape Town EBPR process, the percentage of Rhodocyclus-related cells that contained polyphosphate was about 20% of the total bacterial population, but these cells represented as much as 73% of the polyphosphate-accumulating organisms (PAOs). In an aerated-anoxic EBPR process, Rhodocyclus-related PAOs were less numerous, accounting for 6% of the total bacterial population and 26% of the total PAO population. In addition, 16S ribosomal DNA sequences 99.9% similar to the sequences of Rhodocyclus-related organisms enriched in acetate-fed bench-scale EBPR reactors were recovered from both full-scale plants. These results confirmed the involvement of Rhodocyclus-related organisms in EBPR and demonstrated their importance in full-scale processes. In addition, the results revealed a significant correlation between the type of EBPR process and the PAO community.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, University of Wisconsin—Madison, Madison, WI 53706. Phone: (608) 263-7783. Fax: (608) 262-5199. E-mail: noguera{at}engr.wisc.edu.

{dagger} Present address: Department of Civil and Environmental Engineering, Arizona State University, Tempe, AZ 85287.

{ddagger} Present address: Department of Environmental Engineering, Daejin University, Pocheon, Korea.

§ Present address: Department of Environment Engineering, National Chung-Hsing University, Taichung, Taiwan.

Applied and Environmental Microbiology, June 2002, p. 2763-2769, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.2763-2769.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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