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Applied and Environmental Microbiology, June 2002, p. 3121-3125, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3121-3125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Use of the Verrucomicrobia-Specific Probe EUB338-III and Fluorescent In Situ Hybridization for Detection of "Candidatus Xiphinematobacter" Cells in Nematode Hosts

Tom T. M. Vandekerckhove,1,,2* August Coomans,2 Karen Cornelis,3 Philippe Baert,4 and Monique Gillis1

Laboratorium voor Microbiologie (Vakgroep Biochemie, Fysiologie en Microbiologie),1 Vakgroep Biologie,2 Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie,3 Laboratory for Biochemistry and Molecular Cytology, Ghent University, B-9000 Ghent, Belgium4

Received 9 October 2001/ Accepted 28 February 2002

Fluorescent in situ hybridization with a 16S rRNA probe specific for Verrucomicrobia was used to (i) confirm the division-level identity of and (ii) study the behavior of the obligate intracellular verrucomicrobium "Candidatus Xiphinematobacter" in its nematode hosts. Endosymbionts in the egg move to the pole where the gut primordium arises; hence, they populate the intestinal epithelia of juvenile worms. During the host's molt to adult female, the endosymbionts concentrate around the developing ovaries to occupy the ovarian wall. Some bacteria are enclosed in the ripening oocytes for vertical transmission. Verrucomicrobia in males stay outside the testes because the tiny spermatozoids are not suitable for transmission of cytoplasmic bacteria.

* Corresponding author. Mailing address: Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: 32 9 2648730. Fax: 32 9 2645092. E-mail: tvdkerck{at}yahoo.se.

Applied and Environmental Microbiology, June 2002, p. 3121-3125, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3121-3125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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