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Applied and Environmental Microbiology, June 2002, p. 3126-3128, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3126-3128.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Characterization of Cholylglycine Hydrolase from a Bile-Adapted Strain of Xanthomonas maltophilia and Its Application for Quantitative Hydrolysis of Conjugated Bile Salts{dagger}

Mariangela Dean,1 Carlo Cervellati,1 Elena Casanova,2 Monica Squerzanti,1 Vincenzo Lanzara,1 Alessandro Medici,2 Patrizia Polverino de Laureto,3 and Carlo M. Bergamini1,4*

Department of Biochemistry and Molecular Biology,1 Department of Chemistry,2 ICSI, Centre for the Study of Inflammatory Diseases, University of Ferrara, 44100 Ferrara,4 CRIBI, Biotechnology Centre, University of Padua, Padua, Italy3

Received 26 October 2001/ Accepted 1 March 2002

Purified bile salt hydrolase from bile-adapted Xanthomonas maltophilia displays Michaelis-Menten kinetics on cholylglycine and cholyltaurine and hydrolyzes bile salts also in crude bovine bile. The protein is a dimer and is resistant to proteinases and to heating at 55 to 60°C for up to 60 min, in agreement with calorimetric data.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Ferrara, Via Borsari 46, 44100 Ferrara, Italy. Phone: 0039-0532-291425. Fax: 0039-0532-202723. E-mail: bgc{at}dns.unife.it.

{dagger} This report is dedicated to the memory of our teacher, Mario Rippa, who suddenly died on 27 March 2001.

Applied and Environmental Microbiology, June 2002, p. 3126-3128, Vol. 68, No. 6
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.6.3126-3128.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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