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Applied and Environmental Microbiology, July 2002, p. 3345-3351, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3345-3351.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

In Situ Transfer of Antibiotic Resistance Genes from Transgenic (Transplastomic) Tobacco Plants to Bacteria

Elisabeth Kay,1 Timothy M. Vogel,1 Frank Bertolla,1 Renaud Nalin,2 and Pascal Simonet1*

Ecologie Microbienne, UMR CNRS 5557, Université Claude Bernard, Lyon 1,,1 LibraGen, 69622 Villeurbanne Cedex, France2

Received 14 February 2002/ Accepted 25 April 2002

Interkingdom gene transfer is limited by a combination of physical, biological, and genetic barriers. The results of greenhouse experiments involving transplastomic plants (genetically engineered chloroplast genomes) cocolonized by pathogenic and opportunistic soil bacteria demonstrated that these barriers could be eliminated. The Acinetobacter sp. strain BD413, which is outfitted with homologous sequences to chloroplastic genes, coinfected a transplastomic tobacco plant with Ralstonia solanacearum and was transformed by the plant's transgene (aadA) containing resistance to spectinomycin and streptomycin. However, no transformants were observed when the homologous sequences were omitted from the Acinetobacter sp. strain. Detectable gene transfer from these transgenic plants to bacteria were dependent on gene copy number, bacterial competence, and the presence of homologous sequences. Our data suggest that by selecting plant transgene sequences that are nonhomologous to bacterial sequences, plant biotechnologists could restore the genetic barrier to transgene transfer to bacteria.

* Corresponding author. Mailing address: Ecologie Microbienne, UMR 5557, Université Claude Bernard, Lyon I, 43 bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. Phone: 33 4 72 44 82 89. Fax: 33 4 72 43 12 23. E-mail: simonet{at}biomserv.univ-lyon1.fr.

Applied and Environmental Microbiology, July 2002, p. 3345-3351, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3345-3351.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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