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Applied and Environmental Microbiology, July 2002, p. 3651-3654, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3651-3654.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Cloning of a Gene Encoding Raw-Starch-Digesting Amylase from a Cytophaga sp. and Its Expression in Escherichia coli

Chii-Ling Jeang,* Li-Shien Chen, Ming-Yu Chen, and Rong-Jen Shiau

Department of Food Science, National Chung Hsing University, Taichung, Taiwan, Republic of China

Received 24 September 2001/ Accepted 19 April 2002

A raw-starch-digesting amylase (RSDA) gene from a Cytophaga sp. was cloned and sequenced. The predicted protein product contained 519 amino acids and had high amino acid identity to {alpha}-amylases from three Bacillus species. Only one of the Bacillus {alpha}-amylases has raw-starch-digesting capability, however. The RSDA, expressed in Escherichia coli, had properties similar to those of the enzyme purified from the Cytophaga sp.

* Corresponding author. Mailing address: Department of Food Science, National Chung Hsing University, 250 Kuokang Rd., Taichung 402, Taiwan, R.O.C. Phone: 886-422862797. Fax: 886-422862797. E-mail: cljeang{at}nchu.edu.tw.

Applied and Environmental Microbiology, July 2002, p. 3651-3654, Vol. 68, No. 7
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.7.3651-3654.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • van der Kaaij, R. M., Janecek, S., van der Maarel, M. J. E. C., Dijkhuizen, L. (2007). Phylogenetic and biochemical characterization of a novel cluster of intracellular fungal {alpha}-amylase enzymes. Microbiology 153: 4003-4015 [Abstract] [Full Text]  
  • Shiau, R.-J., Hung, H.-C., Jeang, C.-L. (2003). Improving the Thermostability of Raw-Starch-Digesting Amylase from a Cytophaga sp. by Site-Directed Mutagenesis. Appl. Environ. Microbiol. 69: 2383-2385 [Abstract] [Full Text]  


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