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Applied and Environmental Microbiology, August 2002, p. 3948-3955, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.3948-3955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Molecular Cloning and Characterization of the Gene Coding for the Aerobic Azoreductase from Xenophilus azovorans KF46F

Silke Blümel, Hans-Joachim Knackmuss, and Andreas Stolz*

Institut für Mikrobiologie der Universität Stuttgart, 70569 Stuttgart, Germany

Received 22 January 2002/ Accepted 14 May 2002

The gene coding for an aerobic azoreductase was cloned from Xenophilus azovorans KF46F (formerly Pseudomonas sp. strain KF46F), which was previously shown to grow with the carboxylated azo compound 1-(4'-carboxyphenylazo)-2-naphthol (carboxy-Orange II) as the sole source of carbon and energy. The deduced amino acid sequence encoded a protein with a molecular weight of 30,278 and showed no significant homology to amino acid sequences currently deposited at the relevant data bases. A presumed NAD(P)H-binding site was identified in the amino-terminal region of the azoreductase. The enzyme was heterologously expressed in Escherichia coli and the azoreductase activities of resting cells and cell extracts were compared. The results suggested that whole cells of the recombinant E. coli strains were unable to take up sulfonated azo dyes and therefore did not show in vivo azoreductase activity. The turnover of several industrially relevant azo dyes by cell extracts from the recombinant E. coli strain was demonstrated.

* Corresponding author. Mailing address: Institut für Mikrobiologie der Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany. Phone: 0049-711-685-5489. Fax: 0049-711-685-5725. E-mail: andreas.stolz{at}po.uni-stuttgart.de.

Applied and Environmental Microbiology, August 2002, p. 3948-3955, Vol. 68, No. 8
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.8.3948-3955.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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