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Applied and Environmental Microbiology, September 2002, p. 4182-4186, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4182-4186.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Correspondence of High Levels of Beta-Exotoxin I and the Presence of cry1B in Bacillus thuringiensis

Sylvain Espinasse,1 Michel Gohar,1,2 Josette Chaufaux,1 Christophe Buisson,1 Stéphane Perchat,1 and Vincent Sanchis1,3*

Unité de Recherches de Lutte Biologique, INRA La Minière, 78285 Guyancourt,1 Unité de Biochimie Microbienne, Institut Pasteur, 75724 Paris Cedex 15, France,3 Aventis Crop Science, B9000 Ghent, Belgium2

Received 14 February 2002/ Accepted 29 May 2002

Examination of 640 natural isolates of Bacillus thuringiensis showed that the 58 strains (9%) whose supernatants were toxic to Anthonomus grandis (Coleoptera: Curculionidae) produced between 10 and 175 µg of ß-exotoxin I per ml. We also found that 55 (46%) of a sample of 118 strains whose culture supernatants were not toxic to A. grandis nevertheless produced between 2 and 5 µg/ml. However, these amounts of ß-exotoxin I were below the threshold for detectable toxicity against this insect species. Secretion of large amounts of ß-exotoxin I was strongly associated with the presence of cry1B and vip2 genes in the 640 natural B. thuringiensis isolates studied. We concluded that strains carrying cry1B and vip2 genes also possess, on the same plasmid, genetic determinants necessary to promote high levels of production of ß-exotoxin I.

* Corresponding author. Mailing address: Unité de Biochimie Microbienne, Institut Pasteur, 25/28 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 88 12. Fax: 33 1 45 68 89 38. E-mail: vsanchis{at}pasteur.fr.

Applied and Environmental Microbiology, September 2002, p. 4182-4186, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4182-4186.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Espinasse, S., Gohar, M., Lereclus, D., Sanchis, V. (2004). An Extracytoplasmic-Function Sigma Factor Is Involved in a Pathway Controlling {beta}-Exotoxin I Production in Bacillus thuringiensis subsp. thuringiensis Strain 407-1. J. Bacteriol. 186: 3108-3116 [Abstract] [Full Text]  


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