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Applied and Environmental Microbiology, September 2002, p. 4377-4382, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4377-4382.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Influence of Tropolone on Poria placenta Wood Degradation

P. N. Diouf,¹ N. Delbarre,¹ D. Perrin,¹ P. Gérardin,¹ C. Rapin,² J. P. Jacquot,³ and E. Gelhaye^3*

Laboratoire d'Etudes et de Recherches sur le Matériau Bois, UMR INRA 1093,¹ Laboratoire de Chimie du Solide Minérale, UMR CNRS 7555,² Equipe Interactions Arbres Micro-organismes, UMR INRA 1136, Université Henri Poincaré Nancy I, Faculté des Sciences, 54506 Vandoeuvre-lès-Nancy, France³

Received 22 January 2002/ Accepted 17 June 2002

Fenton reactions are believed to play important roles in wood degradation by brown rot fungi. In this context, the effect of tropolone (2-hydroxycyclohepta-2,4,6-trienone), a metal chelator, on wood degradation by Poria placenta was investigated. Tropolone (50 µM) strongly inhibits fungal growth on malt agar, but this inhibition could be relieved by adding iron salts. With an experimental system containing two separate parts, one supplemented with tropolone (100 µM) and the other not, it was shown that the fungus is able to reallocate essential minerals from the area where they are available and also to grow in these conditions on malt-agar in the presence of tropolone. Nevertheless, even in the presence of an external source of metals, P. placenta is not able to attack pine blocks impregnated with tropolone (5 mM). This wood degradation inhibition is related to the presence of the tropolone hydroxyl group, as shown by the use of analogs (cyclohepta-2,4,6-trienone and 2-methoxycyclohepta-2,4,6-trienone). Furthermore, tropolone possesses both weak antioxidative and weak radical-scavenging properties and a strong affinity for ferric ion and is able to inhibit ferric iron reduction by catecholates, lowering the redox potential of the iron couple. These data are consistent with the hypothesis that tropolone inhibits wood degradation by P. placenta by chelating iron present in wood, thus avoiding initiation of the Fenton reaction. This study demonstrates that iron chelators such as tropolone could be also involved in novel and more environmentally benign preservative systems.

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* Corresponding author. Mailing address: UMR INRA 1136, Equipe Interactions Arbres Micro-organismes, Université Henri Poincaré Nancy I, Faculté des Sciences, BP 239, 54506 Vandoeuvre-lès-Nancy, France. Phone: 33 383 68 42 27. Fax: 33 383 91 25 64. E-mail: gelhaye{at}lcb.uhp-nancy.fr.

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Applied and Environmental Microbiology, September 2002, p. 4377-4382, Vol. 68, No. 9
0099-2240/02/$04.00+0     DOI: 10.1128/AEM.68.9.4377-4382.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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