This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pinhassi, J.
Right arrow Articles by Berman, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pinhassi, J.
Right arrow Articles by Berman, T.
Agricola
Right arrow Articles by Pinhassi, J.
Right arrow Articles by Berman, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2003, p. 199-211, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.199-211.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Differential Growth Response of Colony-Forming {alpha}- and {gamma}-Proteobacteria in Dilution Culture and Nutrient Addition Experiments from Lake Kinneret (Israel), the Eastern Mediterranean Sea, and the Gulf of Eilat{dagger}

Jarone Pinhassi* and Tom Berman

The Yigal Allon Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, IL-14102 Tiberias, Israel

Received 2 August 2002/ Accepted 15 October 2002

Even though it is widely accepted that bacterioplankton growth in lakes and marine ecosystems is determined by the trophic status of the systems, knowledge of the relationship between nutrient concentrations and growth of particular bacterial species is almost nonexistent. To address this question, we performed a series of culture experiments with water from Lake Kinneret (Israel), the eastern Mediterranean Sea, and the Gulf of Eilat (northern Red Sea). In the initial water samples, the proportion of CFU was typically <0.002% of the 4',6'-diamidino-2-phenylindole (DAPI) counts. During incubation until the early stationary phase, the proportion of CFU increased to 20% of the DAPI counts and to 2 to 15% of the DAPI counts in unenriched lake water and seawater dilution cultures, respectively. Sequencing of the 16S ribosomal DNA of colony-forming bacteria in these cultures consistently revealed an abundance of {alpha}-proteobacteria, but notable phylogenetic differences were found at the genus level. Marine dilution cultures were dominated by bacteria in the Roseobacter clade, while lake dilution cultures were dominated by bacteria affiliated with the genera Sphingomonas and Caulobacter. In nutrient (glucose, ammonium, phosphate) addition experiments the CFU comprised 20 to 83% of the newly grown cells. In these incubation experiments fast-growing {gamma}-proteobacteria dominated; in the marine experiments primarily different Vibrio and Alteromonas species appeared, while in the lake water experiments species of the genera Shewanella, Aeromonas, and Rheinheimera grew. These results suggest that major, but different, {gamma}-proteobacterial genera in both freshwater and marine environments have a preference for elevated concentrations of nutrients and easily assimilated organic carbon sources but are selectively outcompeted by {alpha}-proteobacteria in the presence of low nutrient concentrations.

* Corresponding author. Present address: Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC), Passeig Marítim de la Barceloneta 37-49, E-08003 Barcelona, Spain. Phone: 34 93 230 96 08. Fax: 34 93 230 95 55. E-mail: jarone{at}cmima.csic.es.

{dagger} A contribution of Israel Oceanographic and Limnological Research.

Applied and Environmental Microbiology, January 2003, p. 199-211, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.199-211.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alonso-Saez, L., Unanue, M., Latatu, A., Azua, I., Ayo, B., Artolozaga, I., Iriberri, J. (2009). Changes in marine prokaryotic community induced by varying types of dissolved organic matter and subsequent grazing pressure. J PLANKTON RES 31: 1373-1383 [Abstract] [Full Text]  
  • Pinhassi, J., Pujalte, M. J., Pascual, J., Gonzalez, J. M., Lekunberri, I., Pedros-Alio, C., Arahal, D. R. (2009). Bermanella marisrubri gen. nov., sp. nov., a genome-sequenced gammaproteobacterium from the Red Sea. Int. J. Syst. Evol. Microbiol. 59: 373-377 [Abstract] [Full Text]  
  • Kaesler, I., Graeber, I., Borchert, M. S., Pape, T., Dieckmann, R., von Dohren, H., Nielsen, P., Lurz, R., Michaelis, W., Szewzyk, U. (2008). Spongiispira norvegica gen. nov., sp. nov., a marine bacterium isolated from the boreal sponge Isops phlegraei. Int. J. Syst. Evol. Microbiol. 58: 1815-1820 [Abstract] [Full Text]  
  • Allers, E., Niesner, C., Wild, C., Pernthaler, J. (2008). Microbes Enriched in Seawater after Addition of Coral Mucus. Appl. Environ. Microbiol. 74: 3274-3278 [Abstract] [Full Text]  
  • Graeber, I., Kaesler, I., Borchert, M. S., Dieckmann, R., Pape, T., Lurz, R., Nielsen, P., von Dohren, H., Michaelis, W., Szewzyk, U. (2008). Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1. Int. J. Syst. Evol. Microbiol. 58: 585-590 [Abstract] [Full Text]  
  • Arahal, D. R., Lekunberri, I., Gonzalez, J. M., Pascual, J., Pujalte, M. J., Pedros-Alio, C., Pinhassi, J. (2007). Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium. Int. J. Syst. Evol. Microbiol. 57: 1000-1006 [Abstract] [Full Text]  
  • Brettar, I., Christen, R., Hofle, M. G. (2006). Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea.. Int. J. Syst. Evol. Microbiol. 56: 2177-2183 [Abstract] [Full Text]  
  • Alonso, C., Pernthaler, J. (2006). Concentration-dependent patterns of leucine incorporation by coastal picoplankton.. Appl. Environ. Microbiol. 72: 2141-2147 [Abstract] [Full Text]  
  • Yokokawa, T., Nagata, T. (2005). Growth and Grazing Mortality Rates of Phylogenetic Groups of Bacterioplankton in Coastal Marine Environments. Appl. Environ. Microbiol. 71: 6799-6807 [Abstract] [Full Text]  
  • Pernthaler, J., Amann, R. (2005). Fate of Heterotrophic Microbes in Pelagic Habitats: Focus on Populations. Microbiol. Mol. Biol. Rev. 69: 440-461 [Abstract] [Full Text]  
  • Simu, K., Holmfeldt, K., Zweifel, U. L., Hagstrom, A. (2005). Culturability and Coexistence of Colony-Forming and Single-Cell Marine Bacterioplankton. Appl. Environ. Microbiol. 71: 4793-4800 [Abstract] [Full Text]  
  • Olapade, O. A., Leff, L. G. (2005). Seasonal Response of Stream Biofilm Communities to Dissolved Organic Matter and Nutrient Enrichments. Appl. Environ. Microbiol. 71: 2278-2287 [Abstract] [Full Text]  
  • Biebl, H., Allgaier, M., Tindall, B. J., Koblizek, M., Lunsdorf, H., Pukall, R., Wagner-Dobler, I. (2005). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. Int. J. Syst. Evol. Microbiol. 55: 1089-1096 [Abstract] [Full Text]  
  • Liu, Y., Gao, W., Wang, Y., Wu, L., Liu, X., Yan, T., Alm, E., Arkin, A., Thompson, D. K., Fields, M. W., Zhou, J. (2005). Transcriptome Analysis of Shewanella oneidensis MR-1 in Response to Elevated Salt Conditions. J. Bacteriol. 187: 2501-2507 [Abstract] [Full Text]  
  • Pinhassi, J., Sala, M. M., Havskum, H., Peters, F., Guadayol, OÒs., Malits, A., Marrase, C. (2004). Changes in Bacterioplankton Composition under Different Phytoplankton Regimens. Appl. Environ. Microbiol. 70: 6753-6766 [Abstract] [Full Text]  
  • Thompson, J. R., Randa, M. A., Marcelino, L. A., Tomita-Mitchell, A., Lim, E., Polz, M. F. (2004). Diversity and Dynamics of a North Atlantic Coastal Vibrio Community. Appl. Environ. Microbiol. 70: 4103-4110 [Abstract] [Full Text]  
  • Brinkhoff, T., Bach, G., Heidorn, T., Liang, L., Schlingloff, A., Simon, M. (2004). Antibiotic Production by a Roseobacter Clade-Affiliated Species from the German Wadden Sea and Its Antagonistic Effects on Indigenous Isolates. Appl. Environ. Microbiol. 70: 2560-2565 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»