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Applied and Environmental Microbiology, January 2003, p. 358-366, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.358-366.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Secretion of Active-Form Streptoverticillium mobaraense Transglutaminase by Corynebacterium glutamicum: Processing of the Pro-Transglutaminase by a Cosecreted Subtilisin-Like Protease from Streptomyces albogriseolus

Yoshimi Kikuchi,^* Masayo Date, Kei-ichi Yokoyama, Yukiko Umezawa, and Hiroshi Matsui

Institute of Life Sciences, Ajinomoto Co., Inc., Kawasaki 210-8681, Japan

Received 31 July 2002/ Accepted 15 October 2002

The transglutaminase secreted by Streptoverticillium mobaraense is a useful enzyme in the food industry. A fragment of transglutaminase was secreted by Corynebacterium glutamicum when it was coupled on a plasmid to the promoter and signal peptide of a cell surface protein from C. glutamicum. We analyzed the signal peptide and the pro-domain of the transglutaminase gene and found that the signal peptide consists of 31 amino acid residues and the pro-domain consists of 45 residues. When the pro-domain of the transglutaminase was used, the pro-transglutaminase was secreted efficiently by C. glutamicum but had no enzymatic activity. However, when the plasmid carrying the S. mobaraense transglutaminase also encoded SAM-P45, a subtilisin-like serine protease derived from Streptomyces albogriseolus, the peptide bond to the C side of 41-Ser of the pro-transglutaminase was hydrolyzed, and the pro-transglutaminase was converted to an active form. Our findings suggest that C. glutamicum has potential as a host for industrial-scale protein production.

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* Corresponding author. Mailing address: Institute of Life Sciences, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210-8681, Japan. Phone: (81) 44-244-7123. Fax: (81) 44-222-0129. E-mail: yoshimi_kikuchi{at}ajinomoto.com.

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Applied and Environmental Microbiology, January 2003, p. 358-366, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.358-366.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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