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Applied and Environmental Microbiology, January 2003, p. 649-653, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.649-653.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Spoilage Yeast Zygosaccharomyces bailii Forms Mitotic Spores: a Screening Method for Haploidization

Fernando Rodrigues,1,2,3 Paula Ludovico,2 Maria João Sousa,2 H. Yde Steensma,3,4 Manuela Côrte-Real,2* and Cecília Leão1,2

Instituto de Ciências da Vida e Saúde,1 Centro de Ciências do Ambiente, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal,2 Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, 2333 AL Leiden,3 Kluyver Laboratory for Biotechnology, Delft University of Technology, 2628 BC Delft, The Netherlands4

Received 16 July 2002/ Accepted 1 October 2002

Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of Z. bailii. This new set of Z. bailii strains allows the easy isolation of recessive mutants and is suitable for further molecular genetic studies.

* Corresponding author. Mailing address: Departamento de Biologia, Universidade do Minho, 4710-057 Braga, Portugal. Phone: 351 253 604314. Fax: 351 253 678980. E-mail: mcortereal{at}bio.uminho.pt.

Applied and Environmental Microbiology, January 2003, p. 649-653, Vol. 69, No. 1
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.1.649-653.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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