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Applied and Environmental Microbiology, October 2003, p. 6073-6081, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6073-6081.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Diversity of Sulfate-Reducing Bacteria from Two Different Continental Margin Habitats

Xueduan Liu,1,2 Christopher E. Bagwell,1,{dagger} Liyou Wu,1 Allan H. Devol,3 and Jizhong Zhou1*

Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831,1 Department of Plant Pathology, Hunan Agricultural University, Changsha, Hunan, People's Republic of China,2 School of Oceanography, University of Washington, Seattle, Washington 981953

Received 24 March 2003/ Accepted 30 July 2003

This study examined the natural diversity and distributions of sulfate-reducing bacteria along a natural carbon gradient extending down the shelf-slope transition zone of the eastern Pacific continental margin. Dissimilatory (bi)sulfite reductase gene sequences (dsrAB) were PCR amplified and cloned from five different sampling sites, each at a discrete depth, from two different margin systems, one off the Pacific coast of Mexico and another off the coast of Washington State. A total of 1,762 clones were recovered and evaluated by restriction fragment length polymorphism (RFLP) analysis. The majority of the gene sequences recovered showed site and depth restricted distributions; however, a limited number of gene sequences were widely distributed within and between the margin systems. Cluster analysis identified 175 unique RFLP patterns, and nucleotide sequences were determined for corresponding clones. Several different continental margin DsrA sequences clustered with those from formally characterized taxa belonging to the delta subdivision of the class Proteobacteria (Desulfobulbus propionicus, Desulfosarcina variabilis) and the Bacillus-Clostridium (Desulfotomaculum putei) divisions, although the majority of the recovered sequences were phylogenetically divergent relative to all of the other DsrA sequences available for comparison. This study revealed extensive new genetic diversity among sulfate-reducing bacteria in continental margin sedimentary habitats, which appears to be tightly coupled to slope depth, specifically carbon bioavailability.

* Corresponding author. Mailing address: Environmental Sciences Division, Oak Ridge National Laboratory, Bldg. 1505 MS 6038, Bethel Valley Rd., Oak Ridge, TN 37831-6038. Phone: (865) 576-7544. Fax: (865) 576-8646. E-mail: zhouJ{at}ornl.gov.

{dagger} Present address: Westinghouse Savannah River Company, Savannah River Site, Aiken, SC 29808.

Applied and Environmental Microbiology, October 2003, p. 6073-6081, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6073-6081.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wu, L., Kellogg, L., Devol, A. H., Tiedje, J. M., Zhou, J. (2008). Microarray-Based Characterization of Microbial Community Functional Structure and Heterogeneity in Marine Sediments from the Gulf of Mexico. Appl. Environ. Microbiol. 74: 4516-4529 [Abstract] [Full Text]  
  • Dar, S. A., Yao, L., van Dongen, U., Kuenen, J. G., Muyzer, G. (2007). Analysis of Diversity and Activity of Sulfate-Reducing Bacterial Communities in Sulfidogenic Bioreactors Using 16S rRNA and dsrB Genes as Molecular Markers. Appl. Environ. Microbiol. 73: 594-604 [Abstract] [Full Text]  
  • Liebich, J., Schadt, C. W., Chong, S. C., He, Z., Rhee, S.-K., Zhou, J. (2006). Improvement of Oligonucleotide Probe Design Criteria for Functional Gene Microarrays in Environmental Applications. Appl. Environ. Microbiol. 72: 1688-1691 [Abstract] [Full Text]  


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