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Applied and Environmental Microbiology, October 2003, p. 6294-6298, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6294-6298.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Single-Cell Enumeration of an Uncultivated TM7 Subgroup in the Human Subgingival Crevice

Cleber C. Ouverney,1* Gary C. Armitage,2 and David A. Relman1,3,4

Departments of Microbiology and Immunology,1 Medicine, Stanford University, Stanford, California 94305,4 Veterans Affairs Palo Alto Health Care System, Palo Alto, California 94304,3 Department of Stomatology, University of California, San Francisco, California 941432

Received 25 March 2003/ Accepted 9 July 2003

Specific oligonucleotide hybridization conditions were established for single-cell enumeration of uncultivated TM7 and IO25 bacteria by using clones expressing heterologous 16S rRNA. In situ analysis of human subgingival crevice specimens revealed that a greater proportion of samples from sites of chronic periodontitis than from healthy sites contained TM7 subgroup IO25. In addition, IO25 bacterial cells from periodontitis site samples were more abundant and fourfold longer than IO25 cells from healthy site samples.

* Corresponding author. Mailing address: VA Palo Alto Health Care System, Palo Alto, CA 94304. Phone: (650) 493-5000, ext. 63163. Fax: (650) 852-3291. E-mail: ouverney{at}stanford.edu.

Applied and Environmental Microbiology, October 2003, p. 6294-6298, Vol. 69, No. 10
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.10.6294-6298.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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