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Applied and Environmental Microbiology, November 2003, p. 6399-6404, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6399-6404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Bacterial {gamma}-Glutamyltranspeptidase for Enzymatic Synthesis of {gamma}-D-Glutamyl Compounds

Hideyuki Suzuki,* Shunsuke Izuka, Hiromichi Minami, Nobukazu Miyakawa, Sayaka Ishihara, and Hidehiko Kumagai

Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

Received 9 May 2003/ Accepted 7 August 2003

An enzymatic method for synthesizing various {gamma}-D-glutamyl compounds efficiently and stereospecifically involving bacterial {gamma}-glutamyltranspeptidase (EC 2.3.2.2) with D-glutamine as a {gamma}-glutamyl donor was developed. With D-glutamine as a {gamma}-glutamyl donor instead of L-glutamine in {gamma}-glutamyltaurine synthesis, by-products such as {gamma}-glutamylglutamine and {gamma}-glutamyl-{gamma}-glutamyltaurine were not synthesized and the yield of {gamma}-glutamyltaurine dramatically increased from 25 to 71%. It was also shown that the purification could be simplified without these {gamma}-glutamyl by-products. The possibility of synthesizing various {gamma}-D-glutamyl compounds was also shown.

* Corresponding author. Mailing address: Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan. Phone: 81-75-753-6278. Fax: 81-75-753-6275. E-mail: hideyuki{at}lif.kyoto-u.ac.jp.

Applied and Environmental Microbiology, November 2003, p. 6399-6404, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6399-6404.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kurihara, S., Oda, S., Tsuboi, Y., Kim, H. G., Oshida, M., Kumagai, H., Suzuki, H. (2008). {gamma}-Glutamylputrescine Synthetase in the Putrescine Utilization Pathway of Escherichia coli K-12. J. Biol. Chem. 283: 19981-19990 [Abstract] [Full Text]  
  • Suzuki, H., Koyanagi, T., Izuka, S., Onishi, A., Kumagai, H. (2005). The yliA, -B, -C, and -D Genes of Escherichia coli K-12 Encode a Novel Glutathione Importer with an ATP-Binding Cassette. J. Bacteriol. 187: 5861-5867 [Abstract] [Full Text]  
  • Kurihara, S., Oda, S., Kato, K., Kim, H. G., Koyanagi, T., Kumagai, H., Suzuki, H. (2005). A Novel Putrescine Utilization Pathway Involves {gamma}-Glutamylated Intermediates of Escherichia coli K-12. J. Biol. Chem. 280: 4602-4608 [Abstract] [Full Text]  


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