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Applied and Environmental Microbiology, November 2003, p. 6932-6934, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6932-6934.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Influence of Storage on Monodispersed Cells of Mycobacterium terrae Used for Quantitative Carrier Test prEN 14563

Eva Woelk,1 Peter Goroncy-Bermes,2,{dagger}* and Wolfgang Sand1

Institute of Botany, Department of Microbiology, University of Hamburg, D-22602 Hamburg,1 Research and Development, Schülke & Mayr GmbH, D-22851 Norderstedt, Germany2

Received 16 April 2003/ Accepted 26 August 2003

The degree of cell clumping increased with time of storage (1% cell clumps immediately after homogenization and 3 and 6.5% after 48 and 96 h of storage, respectively), and the number of living single cells decreased. Quantitative carrier tests were carried out with these cells using ortho-phthaldialdehyde (OPA) and coco fatty aminoxethylate as biocides. In contrast to OPA, with coco fatty aminoxethylate the reductions obtained with freshly homogenized mycobacteria were significantly higher (P = 0.02) than those obtained with mycobacteria kept in the refrigerator for 4 days. Therefore, it is advisable to prepare the test suspension freshly for each test.

* Corresponding author. Mailing address: Research and Development, Schülke & Mayr GmbH, Robert-Koch-Str. 2, D-22851 Norderstedt, Germany. Phone: 49 40 52100552. Fax: 49 40 52100577. E-mail: peter.goroncy-bermes{at}schuelke-mayr.com.

{dagger} Present address: Division of Innate Immunity, Department of Immunology and Cell Biology, Research Center Borstel, D-23845 Borstel, Germany.

Applied and Environmental Microbiology, November 2003, p. 6932-6934, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6932-6934.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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