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Applied and Environmental Microbiology, November 2003, p. 6943-6945, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6943-6945.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Development of a Synthetic Minimal Medium for Listeria monocytogenes

Hsiang-Ning Tsai{dagger} and David A. Hodgson*

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom

Received 22 April 2003/ Accepted 18 August 2003

A defined solid and liquid minimal medium, HTM, which contained methionine and cysteine as the sole amino acids, was developed for Listeria monocytogenes. Complex broth-grown L. monocytogenes had to adapt to HTM by inducing amino acid biosyntheis. HTM is the simplest minimal medium available for growth of L. monocytogenes.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom. Phone: 44 (0)24 7652 3559. Fax: 44 (0)24 7652 3701. E-mail: David.Hodgson{at}warwick.ac.uk.

{dagger} Present address: Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.

Applied and Environmental Microbiology, November 2003, p. 6943-6945, Vol. 69, No. 11
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.11.6943-6945.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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