This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Faruque, S. M.
Right arrow Articles by Sack, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Faruque, S. M.
Right arrow Articles by Sack, D. A.
Agricola
Right arrow Articles by Faruque, S. M.
Right arrow Articles by Sack, D. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2003, p. 7028-7031, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7028-7031.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh

Shah M. Faruque,1* Nityananda Chowdhury,1 Rasel Khan,1 M. Rubayet Hasan,1 Jebun Nahar,1 M. Johirul Islam,1 Shinji Yamasaki,2 A. N. Ghosh,3 G. Balakrish Nair,1 and David A. Sack1

Molecular Genetics Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, Bangladesh,1 Department of Veterinary Sciences, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai-shi, Osaka 599-8531, Japan,2 National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta 700 010, India3

Received 8 July 2003/ Accepted 18 September 2003

Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface water samples from Bangladesh that specifically lyses strains of S. dysenteriae type 1. This phage, designated SF-9, belongs to the Podoviridae family and has a 41-kb double-stranded DNA genome. Further screening of water samples for the prevalence of the phage revealed 9 of 71 (12.6%) water samples which were positive for the phage. These water samples were also positive in PCR assays for one or more S. dysenteriae type 1-specific genes, including ipaBCD and stx1, and live S. dysenteriae type 1 was isolated from three phage-positive samples. The results of this study suggest that phage SF-9 may have epidemiological applications in tracing the presence of S. dysenteriae type 1 in environmental waters.

* Corresponding author. Mailing address: Molecular Genetics Laboratory, Laboratory Sciences Division, ICDDR,B. GPO Box 128, Dhaka 1000, Bangladesh. Phone: 880 2 8811751 to 880 2 8811760. Fax: 880 2 8812529 and 880 2 8823116. E-mail: faruque{at}icddrb.org.

Applied and Environmental Microbiology, December 2003, p. 7028-7031, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7028-7031.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Garcia-Aljaro, C., Muniesa, M., Jofre, J., Blanch, A. R. (2004). Prevalence of the stx2 Gene in Coliform Populations from Aquatic Environments. Appl. Environ. Microbiol. 70: 3535-3540 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»