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Applied and Environmental Microbiology, December 2003, p. 7545-7548, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7545-7548.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Case Study of the Distribution of Mucosa-Associated Bifidobacterium Species, Lactobacillus Species, and Other Lactic Acid Bacteria in the Human Colon

D. S. Nielsen,1 P. L. Møller,1 V. Rosenfeldt,2 A. Pærregaard,2 K. F. Michaelsen,3 and M. Jakobsen1*

Department of Dairy and Food Science,1 Department of Human Nutrition, Center for Advanced Food Studies, Royal Veterinary and Agricultural University, Frederiksberg,3 University Clinic of Pediatrics, H:S Hvidovre Hospital, Hvidovre,Denmark2

Received 16 April 2003/ Accepted 8 September 2003

The distribution of mucosa-associated bacteria, bifidobacteria and lactobacilli and closely related lactic acid bacteria, in biopsy samples from the ascending, transverse, and descending parts of the colon from four individuals was investigated by denaturing gradient gel electrophoresis (DGGE). Bifidobacterial genus-specific, Lactobacillus group-specific, and universal bacterial primers were used in a nested PCR approach to amplify a fragment of the 16S rRNA gene. DGGE profiles of the bifidobacterial community were relatively simple, with one or two amplicons detected at most sampling sites in the colon. DGGE profiles obtained with Lactobacillus group-specific primers were complex and varied with host and sampling site in the colon. The overall bacterial community varied with host but not sampling site.

* Corresponding author. Mailing address: Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Rolighedsvej 30, 1870 Frederiksberg C, Denmark. Phone: 45 35 28 32 16. Fax: 45 35 28 32 14. E-mail: moj{at}kvl.dk.

Applied and Environmental Microbiology, December 2003, p. 7545-7548, Vol. 69, No. 12
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.12.7545-7548.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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