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Applied and Environmental Microbiology, February 2003, p. 1181-1186, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1181-1186.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Specific Detection of Arcobacter and Campylobacter Strains in Water and Sewage by PCR and Fluorescent In Situ Hybridization

Yolanda Moreno,1 Salut Botella,1 José Luis Alonso,2 María A. Ferrús,1 Manuel Hernández,1 and Javier Hernández1*

Departamento de Biotecnología,1 Instituto de Hidrología y Medio Natural, Universidad Politécnica, 46022 Valencia, Spain2

Received 25 March 2002/ Accepted 1 November 2002

The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media. The detection range of PCR and FISH assays varied between 1 cell/ml (after enrichment) to 103 cells/ml (without enrichment). According to our results, both rRNA-based techniques have the potential to be used as quick and sensitive methods for detection of campylobacters in environmental samples.

* Corresponding author. Mailing address: Departamento de Biotecnología, Universidad Politécnica, Camino de Vera 14, 46022 Valencia, Spain. Phone: 34963877423. Fax: 34963879429. E-mail: jhernand{at}btc.upv.es.

Applied and Environmental Microbiology, February 2003, p. 1181-1186, Vol. 69, No. 2
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.2.1181-1186.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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