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Applied and Environmental Microbiology, March 2003, p. 1623-1628, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1623-1628.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Improved Anaerobic Use of Arginine by Saccharomyces cerevisiae{dagger}

Olga Martin,1 Marjorie C. Brandriss,2 Gisbert Schneider,3 and Alan T. Bakalinsky1*

Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331-6602,1 Department of Microbiology and Molecular Genetics, International Center for Public Health, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101-1709,2 MODLAB Group, Institut für Organische Chemie, Johann Wolfgang Goethe-Universität, D-60439 Frankfurt, Germany3

Received 8 July 2002/ Accepted 12 December 2002

Anaerobic arginine catabolism in Saccharomyces cerevisiae was genetically modified to allow assimilation of all four rather than just three of the nitrogen atoms in arginine. This was accomplished by bypassing normal formation of proline, an unusable nitrogen source in the absence of oxygen, and causing formation of glutamate instead. A pro3 ure2 strain expressing a PGK1 promoter-driven PUT2 allele encoding {Delta}1-pyrroline-5-carboxylate dehydrogenase lacking a mitochondrial targeting sequence produced significant cytoplasmic activity, accumulated twice as much intracellular glutamate, and produced twice as much cell mass as the parent when grown anaerobically on limiting arginine as sole nitrogen source.

* Corresponding author. Mailing address: Department of Food Science and Technology, Wiegand Hall, Oregon State University, Corvallis, OR 97331-6602. Phone: (541) 737-6510. Fax: (541) 737-1877. E-mail: alan.bakalinsky{at}orst.edu.

{dagger} Technical paper no. 11913 of the Oregon Agricultural Experiment Station.

Applied and Environmental Microbiology, March 2003, p. 1623-1628, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1623-1628.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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