This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nuanualsuwan, S. Articles by Cliver, D. O. Search for Related Content PubMed PubMed Citation Articles by Nuanualsuwan, S. Articles by Cliver, D. O. Agricola Articles by Nuanualsuwan, S. Articles by Cliver, D. O.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2003, p. 1629-1632, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1629-1632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Infectivity of RNA from Inactivated Poliovirus

Suphachai Nuanualsuwan and Dean O. Cliver^*

World Health Organization Collaborating Center for Food Virology, Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 95616-8743

Received 30 July 2002/ Accepted 10 December 2002

During inactivation of poliovirus type 1 (PV-1) by exposure to UV, hypochlorite, and heat (72°C), the infectivity of the virus was compared with that of its RNA. DEAE-dextran (1-mg/ml concentration in Dulbecco's modified Eagle medium buffered with 0.05 M Tris, pH 7.4) was used to facilitate transfecting PV-1 RNA into FRhK-4 host cells. After interaction of PV-1 RNA with cell monolayer at room temperature (21 to 22°C) for 20 min, the monolayers were washed with 5 ml of Hanks balanced salt solution. The remainder of the procedure was the same as that for the conventional plaque technique, which was also used for quantifying the PV-1 whole-particle infectivity. Plaque formation by extracted RNA was approximately 100,000-fold less efficient than that by whole virions. The slopes of best-fit regression lines of inactivation curves for virion infectivity and RNA infectivity were compared to determine the target of inactivation. For UV and hypochlorite inactivation the slopes of inactivation curves of virion infectivity and RNA infectivity were not statistically different. However, the difference of slopes of inactivation curves of virion infectivity and RNA infectivity was statistically significant for thermal inactivation. The results of these experiments indicate that viral RNA is a primary target of UV and hypochlorite inactivations but that the sole target of thermal inactivation is the viral capsid.

---

* Corresponding author. Mailing address: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616-8743. Phone: (530) 754-9120. Fax: (530) 752-5845. E-mail: docliver{at}ucdavis.edu.

---

Applied and Environmental Microbiology, March 2003, p. 1629-1632, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1629-1632.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Pecson, B. M., Martin, L. V., Kohn, T. (2009). Quantitative PCR for Determining the Infectivity of Bacteriophage MS2 upon Inactivation by Heat, UV-B Radiation, and Singlet Oxygen: Advantages and Limitations of an Enzymatic Treatment To Reduce False-Positive Results. Appl. Environ. Microbiol. 75: 5544-5554 [Abstract] [Full Text]  
• Sirikanchana, K., Shisler, J. L., Marinas, B. J. (2008). Effect of Exposure to UV-C Irradiation and Monochloramine on Adenovirus Serotype 2 Early Protein Expression and DNA Replication. Appl. Environ. Microbiol. 74: 3774-3782 [Abstract] [Full Text]  
• Kamolsiripichaiporn, S., Subharat, S., Udon, R., Thongtha, P., Nuanualsuwan, S. (2007). Thermal Inactivation of Foot-and-Mouth Disease Viruses in Suspension. Appl. Environ. Microbiol. 73: 7177-7184 [Abstract] [Full Text]